MetaCyc Pathway: superpathway of ammonia assimilation (plants) in Pseudomonas fluorescens FW300-N2E2

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Reactions and Genes 10% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine
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Glutamate--ammonia ligase:
ammonium + L-glutamate + ATP→L-glutamine + ADP + H+ + phosphate (EC 6.3.1.2)
Pf6N2E2_2177 +0.3
Pf6N2E2_2730 +0.1
Pf6N2E2_3821 N.D.
Pf6N2E2_4509 -4.0
Pf6N2E2_4511 -3.7
Pf6N2E2_5686 +0.1
Glutamate synthase (ferredoxin) (in reverse):
L-glutamine + 2-oxoglutarate + 2 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→2 L-glutamate + 2 an oxidized ferredoxin [iron-sulfur] cluster (EC 1.4.7.1)
Pf6N2E2_5461 +0.0
Glutamate synthase (NADH) (in reverse):
L-glutamine + 2-oxoglutarate + NADH + H+→2 L-glutamate + NAD+ (EC 1.4.1.14)
Pf6N2E2_3751 +0.0
Pf6N2E2_497 -0.2

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information