MetaCyc Pathway: oleate β-oxidation in Pseudomonas fluorescens SBW25-INTG

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Reactions and Genes p-Coumaric (C) and Ammonium chloride (N); with MOPS
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Long-chain-fatty-acid--CoA ligase:
oleate + ATP + coenzyme A→oleoyl-CoA + AMP + diphosphate (EC 6.2.1.3)
PFLU_RS17185 +0.2
PFLU_RS21255 +0.1
PFLU_RS23680 -3.8
PFLU_RS23685 +0.0
oleoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(2E,9Z)-octadec-2,9-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.8)
PFLU_RS17205 -0.3
Enoyl-CoA hydratase (in reverse):
(2E,9Z)-octadec-2,9-enoyl-CoA + H2O→(S)-3-hydroxy-(9Z)-octadecenoyl-CoA (EC 4.2.1.17)
PFLU_RS07105 -0.0
PFLU_RS07650 -4.7
PFLU_RS08485 +0.0
PFLU_RS10835 +0.0
PFLU_RS12025 +0.1
PFLU_RS13550 -0.3
PFLU_RS14775 -0.4
PFLU_RS14785 +0.2
PFLU_RS16080 -3.9
PFLU_RS22820 -0.1
PFLU_RS22840 +0.0
PFLU_RS24260 -0.1
3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxy-(9Z)-octadecenoyl-CoA + NAD+→3-oxo-(9Z)-octadecenoyl-CoA + NADH + H+ (EC 1.1.1.35)
PFLU_RS07650 -4.7
PFLU_RS11090 -0.2
PFLU_RS22840 +0.0
Acetyl-CoA C-acyltransferase (in reverse):
3-oxo-(9Z)-octadecenoyl-CoA + coenzyme A→acetyl-CoA + (7Z)-hexadecenoyl-CoA (EC 2.3.1.16)
PFLU_RS03225 -0.0
PFLU_RS06735 -4.6
PFLU_RS07655 -4.7
PFLU_RS10575 -0.1
PFLU_RS14765 -0.1
PFLU_RS16065 +0.2
PFLU_RS17190 -0.5
PFLU_RS21180 -0.0
PFLU_RS22845 -0.3
(7Z)-hexadecenoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(2E,7Z)-hexadec-2,7-dienoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.8)
PFLU_RS17205 -0.3
Enoyl-CoA hydratase (in reverse):
(2E,7Z)-hexadec-2,7-dienoyl-CoA + H2O→(S)-3-hydroxy-(7Z)-hexadecenoyl-CoA (EC 4.2.1.17)
PFLU_RS07105 -0.0
PFLU_RS07650 -4.7
PFLU_RS08485 +0.0
PFLU_RS10835 +0.0
PFLU_RS12025 +0.1
PFLU_RS13550 -0.3
PFLU_RS14775 -0.4
PFLU_RS14785 +0.2
PFLU_RS16080 -3.9
PFLU_RS22820 -0.1
PFLU_RS22840 +0.0
PFLU_RS24260 -0.1
3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxy-(7Z)-hexadecenoyl-CoA + NAD+→3-oxo-(7Z)-hexadecenoyl-CoA + NADH + H+ (EC 1.1.1.35)
PFLU_RS07650 -4.7
PFLU_RS11090 -0.2
PFLU_RS22840 +0.0
Acetyl-CoA C-acyltransferase (in reverse):
3-oxo-(7Z)-hexadecenoyl-CoA + coenzyme A→acetyl-CoA + (5Z)-tetradecenoyl-CoA (EC 2.3.1.16)
PFLU_RS03225 -0.0
PFLU_RS06735 -4.6
PFLU_RS07655 -4.7
PFLU_RS10575 -0.1
PFLU_RS14765 -0.1
PFLU_RS16065 +0.2
PFLU_RS17190 -0.5
PFLU_RS21180 -0.0
PFLU_RS22845 -0.3
(5Z)-tetradecenoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(2E,5Z)-tetradec-2,5-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.8)
PFLU_RS17205 -0.3
Dodecenoyl-CoA isomerase:
(2E,5Z)-tetradec-2,5-enoyl-CoA→(3E,5Z)-tetradeca-3,5-dienoyl-CoA (EC 5.3.3.8)
PFLU_RS07650 -4.7
Enoyl-CoA hydratase (in reverse):
(2E,5Z)-tetradec-2,5-enoyl-CoA + H2O→(S)-3-hydroxy-(5Z)-tetradecenoyl-CoA (EC 4.2.1.17)
PFLU_RS07105 -0.0
PFLU_RS07650 -4.7
PFLU_RS08485 +0.0
PFLU_RS10835 +0.0
PFLU_RS12025 +0.1
PFLU_RS13550 -0.3
PFLU_RS14775 -0.4
PFLU_RS14785 +0.2
PFLU_RS16080 -3.9
PFLU_RS22820 -0.1
PFLU_RS22840 +0.0
PFLU_RS24260 -0.1
Long-chain-3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxy-(5Z)-tetradecenoyl-CoA + NAD+→3-oxo-(5Z)-tetradecenoyl-CoA + NADH + H+ (EC 1.1.1.211; 1.1.1.35)
PFLU_RS07650 -4.7
PFLU_RS11090 -0.2
PFLU_RS22840 +0.0
(3E,5Z)-tetradeca-3,5-dienoyl-CoA + H2O→(3E,5Z)-tetradeca-3,5-dienoate + coenzyme A + H+
PFLU_RS26405 -0.5
Acetyl-CoA C-acyltransferase (in reverse):
3-oxo-(5Z)-tetradecenoyl-CoA + coenzyme A→acetyl-CoA + (3Z)-dodec-3-enoyl-CoA (EC 2.3.1.16)
PFLU_RS03225 -0.0
PFLU_RS06735 -4.6
PFLU_RS07655 -4.7
PFLU_RS10575 -0.1
PFLU_RS14765 -0.1
PFLU_RS16065 +0.2
PFLU_RS17190 -0.5
PFLU_RS21180 -0.0
PFLU_RS22845 -0.3
Dodecenoyl-CoA isomerase:
(3Z)-dodec-3-enoyl-CoA→(2E)-dodec-2-enoyl-CoA (EC 5.3.3.8)
PFLU_RS07650 -4.7
Long-chain-enoyl-CoA hydratase (in reverse):
(2E)-dodec-2-enoyl-CoA + H2O→(S)-3-hydroxydodecanoyl-CoA (EC 4.2.1.74)
No genes
Long-chain-3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxydodecanoyl-CoA + NAD+→3-oxododecanoyl-CoA + NADH + H+ (EC 1.1.1.211; 1.1.1.35)
PFLU_RS07650 -4.7
PFLU_RS11090 -0.2
PFLU_RS22840 +0.0
Acetyl-CoA C-acyltransferase (in reverse):
3-oxododecanoyl-CoA + coenzyme A→acetyl-CoA + decanoyl-CoA (EC 2.3.1.16)
PFLU_RS03225 -0.0
PFLU_RS06735 -4.6
PFLU_RS07655 -4.7
PFLU_RS10575 -0.1
PFLU_RS14765 -0.1
PFLU_RS16065 +0.2
PFLU_RS17190 -0.5
PFLU_RS21180 -0.0
PFLU_RS22845 -0.3
decanoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(2E)-dec-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.7)
PFLU_RS02225 -0.1
PFLU_RS09195 -0.2
PFLU_RS16060 +0.2
PFLU_RS26520 -0.2
PFLU_RS27605 -2.4
PFLU_RS27610 +0.1
PFLU_RS27620 -3.6
Long-chain-enoyl-CoA hydratase (in reverse):
(2E)-dec-2-enoyl-CoA + H2O→(S)-3-hydroxydecanoyl-CoA (EC 4.2.1.74)
PFLU_RS07105 -0.0
PFLU_RS07650 -4.7
PFLU_RS08485 +0.0
PFLU_RS10835 +0.0
PFLU_RS12025 +0.1
PFLU_RS24260 -0.1
3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxydecanoyl-CoA + NAD+→3-oxodecanoyl-CoA + NADH + H+ (EC 1.1.1.35)
PFLU_RS07650 -4.7
PFLU_RS11090 -0.2
PFLU_RS22840 +0.0
Acetyl-CoA C-acyltransferase (in reverse):
3-oxodecanoyl-CoA + coenzyme A→acetyl-CoA + octanoyl-CoA (EC 2.3.1.16)
PFLU_RS03225 -0.0
PFLU_RS06735 -4.6
PFLU_RS07655 -4.7
PFLU_RS10575 -0.1
PFLU_RS14765 -0.1
PFLU_RS16065 +0.2
PFLU_RS17190 -0.5
PFLU_RS21180 -0.0
PFLU_RS22845 -0.3
octanoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(2E)-oct-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.7)
PFLU_RS02225 -0.1
PFLU_RS16060 +0.2
PFLU_RS26520 -0.2
PFLU_RS27605 -2.4
PFLU_RS27610 +0.1
PFLU_RS27620 -3.6
Long-chain-enoyl-CoA hydratase (in reverse):
(2E)-oct-2-enoyl-CoA + H2O→(3S)-3-hydroxyoctanoyl-CoA (EC 4.2.1.74)
No genes
3-hydroxyacyl-CoA dehydrogenase:
(3S)-3-hydroxyoctanoyl-CoA + NAD+→3-oxooctanoyl-CoA + NADH + H+ (EC 1.1.1.35)
PFLU_RS07650 -4.7
PFLU_RS11090 -0.2
PFLU_RS22840 +0.0
Acetyl-CoA C-acyltransferase (in reverse):
3-oxooctanoyl-CoA + coenzyme A→acetyl-CoA + hexanoyl-CoA (EC 2.3.1.16)
PFLU_RS03225 -0.0
PFLU_RS06735 -4.6
PFLU_RS07655 -4.7
PFLU_RS10575 -0.1
PFLU_RS14765 -0.1
PFLU_RS16065 +0.2
PFLU_RS17190 -0.5
PFLU_RS21180 -0.0
PFLU_RS22845 -0.3
hexanoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(2E)-hex-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.7)
PFLU_RS02225 -0.1
PFLU_RS16060 +0.2
PFLU_RS26520 -0.2
PFLU_RS27605 -2.4
PFLU_RS27610 +0.1
PFLU_RS27620 -3.6
Long-chain-enoyl-CoA hydratase (in reverse):
(2E)-hex-2-enoyl-CoA + H2O→(S)-3-hydroxyhexanoyl-CoA (EC 4.2.1.74)
PFLU_RS07105 -0.0
PFLU_RS07650 -4.7
PFLU_RS08485 +0.0
PFLU_RS10835 +0.0
PFLU_RS12025 +0.1
PFLU_RS24260 -0.1
3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxyhexanoyl-CoA + NAD+→3-oxohexanoyl-CoA + NADH + H+ (EC 1.1.1.35)
PFLU_RS07650 -4.7
PFLU_RS11090 -0.2
PFLU_RS22840 +0.0
Acetyl-CoA C-acyltransferase (in reverse):
3-oxohexanoyl-CoA + coenzyme A→acetyl-CoA + butanoyl-CoA (EC 2.3.1.16; 2.3.1.9)
PFLU_RS03225 -0.0
PFLU_RS06735 -4.6
PFLU_RS07655 -4.7
PFLU_RS10575 -0.1
PFLU_RS14765 -0.1
PFLU_RS16065 +0.2
PFLU_RS17190 -0.5
PFLU_RS21180 -0.0
PFLU_RS22845 -0.3
butanoyl-CoA + an oxidized electron-transfer flavoprotein + H+→crotonyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.1)
PFLU_RS02225 -0.1
crotonyl-CoA + H2O→(S)-3-hydroxybutanoyl-CoA (EC 4.2.1.150)
PFLU_RS07105 -0.0
PFLU_RS07650 -4.7
PFLU_RS08485 +0.0
PFLU_RS10835 +0.0
PFLU_RS12025 +0.1
PFLU_RS24260 -0.1
3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxybutanoyl-CoA + NAD+→acetoacetyl-CoA + NADH + H+ (EC 1.1.1.35)
PFLU_RS07650 -4.7
PFLU_RS11090 -0.2
PFLU_RS22840 +0.0
Acetyl-CoA C-acyltransferase (in reverse):
acetoacetyl-CoA + coenzyme A→2 acetyl-CoA (EC 2.3.1.16; 2.3.1.9)
PFLU_RS03225 -0.0
PFLU_RS06735 -4.6
PFLU_RS07655 -4.7
PFLU_RS10575 -0.1
PFLU_RS14765 -0.1
PFLU_RS16065 +0.2
PFLU_RS17190 -0.5
PFLU_RS21180 -0.0
PFLU_RS22845 -0.3

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information