MetaCyc Pathway: L-asparagine biosynthesis III (tRNA-dependent) in Rhodanobacter denitrificans FW104-10B01

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Reactions and Genes R2A_PIPES pH 4
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Glutamate synthase (NADPH):
L-glutamine + H2O→ammonia + L-glutamate + H+ (EC 1.4.1.13; 1.4.1.14; 1.4.7.1; 2.6.1.85; 3.5.1.2; 3.5.1.38; 4.1.3.27; 4.3.2.10; 4.3.3.6; 6.3.4.2; 6.3.5.13; 6.3.5.2; 6.3.5.4; 6.3.5.5; 6.3.5.6; 6.3.5.7)
LRK54_RS00110 N.D.
LRK54_RS00305 -2.8
LRK54_RS01170 +0.0
LRK54_RS04620 -0.2
LRK54_RS05525 -1.4
LRK54_RS06380 -0.0
LRK54_RS09255 -0.2
LRK54_RS10960 +0.2
LRK54_RS10965 -0.1
LRK54_RS11415 -3.2
LRK54_RS11560 -2.7
LRK54_RS13180 +0.0
LRK54_RS13285 -0.0
LRK54_RS14550 -0.1
LRK54_RS16605 N.D.
LRK54_RS17995 N.D.
LRK54_RS18000 N.D.
Aspartate--tRNA(Asn) ligase:
L-aspartate + tRNAAsn + ATP→an L-aspartyl-[tRNAAsn] + AMP + diphosphate (EC 6.1.1.23)
No genes
Asparaginyl-tRNA synthase (glutamine-hydrolyzing):
an L-aspartyl-[tRNAAsn] + ATP→a 4-phosphooxy-L-aspartyl-[tRNAAsn] + ADP (EC 6.3.5.6)
LRK54_RS05525 -1.4
Asparaginyl-tRNA synthase (glutamine-hydrolyzing):
a 4-phosphooxy-L-aspartyl-[tRNAAsn] + ammonia→an L-asparaginyl-[tRNAAsn] + phosphate (EC 6.3.5.6)
LRK54_RS05525 -1.4

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information