MetaCyc Pathway: zwittermicin A biosynthesis in Pseudomonas fluorescens FW300-N2E2

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Reactions and Genes Cobalt chloride 0.6 mM
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L-serine + ATP + a holo-[L-seryl-carrier protein]→an L-seryl-[L-seryl-carrier protein] + AMP + diphosphate (EC 6.2.1.72)
No genes
3-phospho-D-glyceroyl phosphate + a soluble [acyl-carrier protein] + H2O→a D-glyceryl-[acp] + 2 phosphate
No genes
L-serine + ATP→O-phospho-L-serine + ADP + H+ (EC 2.7.1.225)
No genes
an L-seryl-[L-seryl-carrier protein][cytosol] + NAD+[cytosol]→a 2-aminomalonyl semialdehyde-[L-seryl-carrier protein][cytosol] + NADH[cytosol] + H+[cytosol]
No genes
a D-glyceryl-[acp] + NAD+→a (2R)-2-hydroxy-3-oxopropanoyl-[acp] + NADH + H+
No genes
O-phospho-L-serine + L-glutamate→N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate + phosphate (EC 2.5.1.140)
Pf6N2E2_3321 -1.5
a 2-aminomalonyl semialdehyde-[L-seryl-carrier protein] + FAD + H2O→an aminomalonyl-[L-seryl-carrier protein] + FADH2
No genes
a (2R)-2-hydroxy-3-oxopropanoyl-[acp] + FAD + H2O→a (2R)-2-hydroxymalonyl-[acp] + FADH2 (EC 1.2.8.M1)
No genes
N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate + NAD+ + H2O→L-2,3-diaminopropanoate + 2-oxoglutarate + NADH + H+ (EC 1.5.1.51)
No genes
L-2,3-diaminopropanoate + carbamoyl phosphate→β-ureidoalanine + H+ + phosphate
No genes
a (2R)-2-hydroxymalonyl-[acp] + an aminomalonyl-[L-seryl-carrier protein] + β-ureidoalanine + L-serine + L-asparagine + a fatty acid + L-alanine + malonyl-CoA + 8 NADPH + dioxygen + 12 H+ + a holo acp domain within a [polyketide synthase]→proto-zwittermicin A-L-alanyl-[PKS] + a soluble [acyl-carrier protein] + 2 CO2 + coenzyme A + 8 NADP+ + 9 H2O + a holo-[L-seryl-carrier protein]
No genes
proto-zwittermicin A-L-alanyl-[PKS] + L-leucine + L-methionine + NADPH + dioxygen→proto-zwittermicin A + pyruvoyl-L-leucyl-L-methionine + NADP+ + a holo acp domain within a [polyketide synthase] + 2 H2O
No genes
proto-zwittermicin A + H2O→zwittermicin A + an acyl-D-asparagine
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information