MetaCyc Pathway: trans-4-hydroxy-L-proline degradation I in Pseudomonas fluorescens FW300-N2E2

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Reactions and Genes Cobalt chloride 0.6 mM
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trans-4-hydroxy-L-proline[in] + an electron-transfer quinone→(3R,5S)-3-hydroxy-1-pyrroline-5-carboxylate[in] + an electron-transfer quinol + H+[in] (EC 1.5.5.3)
No genes
(3R,5S)-3-hydroxy-1-pyrroline-5-carboxylate + H+ + H2O→L-4-hydroxyglutamate semialdehyde (spontaneous)
No genes
L-4-hydroxyglutamate semialdehyde + NAD+ + H2O→erythro-4-hydroxy-L-glutamate + NADH + 2 H+
No genes
4-hydroxyglutamate transaminase:
erythro-4-hydroxy-L-glutamate + 2-oxoglutarate→(4R)-4-hydroxy-2-oxoglutarate + L-glutamate (EC 2.6.1.23)
Pf6N2E2_1496 -0.5
Pf6N2E2_2127 -1.5
Pf6N2E2_2446 +0.3
Pf6N2E2_2632 -0.2
Pf6N2E2_3173 -0.1
Pf6N2E2_4288 +0.2
Pf6N2E2_4386 -0.6
Pf6N2E2_462 +0.2
Pf6N2E2_5300 -1.5
Pf6N2E2_5813 -0.6
Pf6N2E2_5890 -0.2
Pf6N2E2_5891 N.D.
Pf6N2E2_5895 -0.1
Pf6N2E2_5989 +2.1
Pf6N2E2_637 +0.3
Pf6N2E2_684 +0.1
4-hydroxy-2-oxoglutarate aldolase:
(4R)-4-hydroxy-2-oxoglutarate→glyoxylate + pyruvate (EC 4.1.3.16)
Pf6N2E2_2883 N.D.

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information