MetaCyc Pathway: indole-3-acetate biosynthesis II in Pseudomonas fluorescens FW300-N2E2

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Reactions and Genes Cobalt chloride 0.6 mM
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L-tryptophan + H+→tryptamine + CO2 (EC 4.1.1.105; 4.1.1.28)
No genes
L-tryptophan + dioxygen + a reduced [NADPH-hemoprotein reductase]→N2-hydroxy-L-tryptophan + an oxidized [NADPH-hemoprotein reductase] + H+ + H2O (EC 1.14.14.156)
No genes
Aspartate transaminase:
L-tryptophan + 2-oxoglutarate→(indol-3-yl)pyruvate + L-glutamate (EC 2.6.1.1; 2.6.1.27)
Pf6N2E2_1496 -0.5
Pf6N2E2_2127 -0.8
Pf6N2E2_2446 +0.0
Pf6N2E2_2632 -0.1
Pf6N2E2_3173 -0.3
Pf6N2E2_4288 +0.3
Pf6N2E2_4386 -0.4
Pf6N2E2_462 +0.1
Pf6N2E2_5300 -1.3
Pf6N2E2_5813 -0.5
Pf6N2E2_5890 -0.1
Pf6N2E2_5891 N.D.
Pf6N2E2_5895 +0.0
Pf6N2E2_5989 +1.7
Pf6N2E2_637 -0.1
Pf6N2E2_684 -0.0
N2-hydroxy-L-tryptophan + dioxygen + a reduced [NADPH-hemoprotein reductase]→N2,N2-dihydroxy-L-tryptophan + an oxidized [NADPH-hemoprotein reductase] + H2O (EC 1.14.14.156)
No genes
tryptamine + NADPH + dioxygen→N-hydroxyl-tryptamine + NADP+ + H2O
No genes
(indol-3-yl)pyruvate + NADPH + dioxygen + H+→(indol-3-yl)acetate + CO2 + NADP+ + H2O (EC 1.14.13.168)
No genes
N-hydroxyl-tryptamine + an oxidized electron carrier→(E)-(indol-3-yl)acetaldehyde oxime + a reduced two electron carrier
No genes
N2,N2-dihydroxy-L-tryptophan + H+→(E)-(indol-3-yl)acetaldehyde oxime + CO2 + H2O (spontaneous) (EC 1.14.14.156)
No genes
(E)-(indol-3-yl)acetaldehyde oxime→(indol-3-yl)acetonitrile + H2O (EC 4.8.1.3)
No genes
Nitrilase:
(indol-3-yl)acetonitrile + 2 H2O→(indol-3-yl)acetate + ammonium (EC 3.5.5.1)
Pf6N2E2_748 -0.5
Nitrile hydratase (in reverse):
(indol-3-yl)acetonitrile + H2O→(indol-3-yl)acetamide (EC 4.2.1.84)
Pf6N2E2_876 +0.3
Pf6N2E2_877 +0.2
Amidase:
(indol-3-yl)acetamide + H2O→(indol-3-yl)acetate + ammonium (EC 3.5.1.4)
Pf6N2E2_1330 +0.1
Pf6N2E2_190 +0.1
Pf6N2E2_3172 +0.3
Pf6N2E2_878 +0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information