MetaCyc Pathway: L-methionine biosynthesis II in Pseudomonas fluorescens FW300-N2E2

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Reactions and Genes Cobalt chloride 0.6 mM
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Homoserine kinase:
L-homoserine + ATP→O-phospho-L-homoserine + ADP + H+ (EC 2.7.1.39)
Pf6N2E2_4164 N.D.
Pf6N2E2_6055 -1.0
Cystathionine gamma-synthase:
O-phospho-L-homoserine + L-cysteine→L-cystathionine + phosphate (EC 2.5.1.48)
Pf6N2E2_4958 -0.2
Pf6N2E2_77 +0.2
Cysteine-S-conjugate beta-lyase:
L-cystathionine→2-aminoprop-2-enoate + L-homocysteine (EC 4.4.1.13)
No genes
5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase:
L-homocysteine + 5-methyltetrahydropteroyl tri-L-glutamate→L-methionine + tetrahydropteroyl tri-L-glutamate (EC 2.1.1.14)
Pf6N2E2_5472 +0.1
Tryptophanase:
2-aminoprop-2-enoate→2-iminopropanoate (spontaneous) (EC 4.1.99.1; 4.1.99.2; 4.3.1.13; 4.3.1.17; 4.3.1.18; 4.3.3.8; 4.4.1.1; 4.4.1.13; 4.4.1.15; 4.4.1.25; 4.4.1.28; 4.4.1.35; 4.5.1.2)
Pf6N2E2_2047 +0.1
Pf6N2E2_3918 +2.1
Pf6N2E2_4685 -0.4
Pf6N2E2_5557 +0.0
Pf6N2E2_5896 -0.2
2-iminopropanoate + H2O→pyruvate + ammonium (spontaneous) (EC 3.5.99.10; 4.1.99.1; 4.1.99.2; 4.3.1.13; 4.3.1.17; 4.3.1.18; 4.3.3.8; 4.4.1.1; 4.4.1.13; 4.4.1.15; 4.4.1.25; 4.4.1.28; 4.4.1.35; 4.5.1.2)
Pf6N2E2_2047 +0.1
Pf6N2E2_3918 +2.1
Pf6N2E2_4360 -2.0
Pf6N2E2_4685 -0.4
Pf6N2E2_5557 +0.0
Pf6N2E2_5896 -0.2

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information