| Reactions and Genes | Nickel (II) chloride 1.5 mM |
|---|
| remove |
5-formyltetrahydrofolate cyclo-ligase:
a (6S)-5-formyltetrahydrofolate + ATP→a 5,10-methenyltetrahydrofolate + ADP + phosphate (EC 6.3.3.2) |
| Pf6N2E2_4476 | -0.5 |
Dihydrofolate reductase (in reverse):
a 7,8-dihydrofolate + NADPH + H+→a tetrahydrofolate + NADP+ (EC 1.5.1.3) |
| Pf6N2E2_4561 | N.D. |
| Pf6N2E2_5274 | +3.6 |
| a tetrahydrofolate + glycine + NAD+→a 5,10-methylenetetrahydrofolate + ammonium + CO2 + NADH (EC 1.4.1.27) |
| Pf6N2E2_4251 | +0.0 |
| Pf6N2E2_4485 | +0.2 |
| Pf6N2E2_5857 | N.D. |
Glycine hydroxymethyltransferase (in reverse):
a 5,10-methylenetetrahydrofolate + glycine + H2O→a tetrahydrofolate + L-serine (EC 2.1.2.1) |
| Pf6N2E2_4717 | +0.1 |
| Pf6N2E2_5082 | N.D. |
| Pf6N2E2_5083 | -0.0 |
| Pf6N2E2_5295 | +0.3 |
Methionine synthase:
a 5-methyltetrahydrofolate + L-homocysteine→a tetrahydrofolate + L-methionine (EC 2.1.1.13) |
| Pf6N2E2_2068 | -0.5 |
Methylenetetrahydrofolate dehydrogenase (NADP(+)):
a 5,10-methylenetetrahydrofolate + NADP+→a 5,10-methenyltetrahydrofolate + NADPH (EC 1.5.1.5) |
| Pf6N2E2_2221 | N.D. |
| a 5,10-methylenetetrahydrofolate + NADH + H+→a 5-methyltetrahydrofolate + NAD+ (EC 1.5.1.54) |
| Pf6N2E2_4638 | -0.8 |
Thymidylate synthase:
a 5,10-methylenetetrahydrofolate + dUMP→a 7,8-dihydrofolate + dTMP (EC 2.1.1.45) |
| Pf6N2E2_3946 | N.D. |
Methenyltetrahydrofolate cyclohydrolase:
a 5,10-methenyltetrahydrofolate + H2O→an N10-formyltetrahydrofolate + H+ (EC 3.5.4.9) |
| Pf6N2E2_2221 | N.D. |
Links:
Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information