MetaCyc Pathway: diacylglycerol and triacylglycerol biosynthesis in Pseudomonas fluorescens FW300-N2E2

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Reactions and Genes Cobalt chloride 0.6 mM
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Glycerol-3-phosphate O-acyltransferase:
sn-glycerol 3-phosphate + an acyl-CoA→a 2-lysophosphatidate + coenzyme A (EC 2.3.1.15)
Pf6N2E2_3087 -0.2
Pf6N2E2_4795 +3.6
Pf6N2E2_5950 +0.4
1-acylglycerol-3-phosphate O-acyltransferase:
a 2-lysophosphatidate + an acyl-CoA→a phosphatidate + coenzyme A (EC 2.3.1.51)
Pf6N2E2_2816 N.D.
Pf6N2E2_4192 N.D.
Pf6N2E2_4950 -0.6
Pf6N2E2_5765 +0.1
Pf6N2E2_6003 -0.0
Phosphatidate phosphatase:
a phosphatidate + H2O→a 1,2-diacyl-sn-glycerol + phosphate (EC 3.1.3.4)
Pf6N2E2_2985 -1.1
Diacylglycerol O-acyltransferase:
a 1,2-diacyl-sn-glycerol + an acyl-CoA→a triacyl-sn-glycerol + coenzyme A (EC 2.3.1.20)
No genes
2 a 1,2-diacyl-sn-glycerol→a triacyl-sn-glycerol + a 2-acylglycerol
No genes
a 1,2-diacyl-sn-glycerol + CTP→a phosphatidate + CDP + H+ (EC 2.7.1.174)
No genes
Phospholipid:diacylglycerol acyltransferase:
a 1,2-diacyl-sn-glycerol + a phosphatidylcholine→a triacyl-sn-glycerol + a 2-lysophosphatidylcholine (EC 2.3.1.158)
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information