MetaCyc Pathway: superpathway of L-methionine biosynthesis (transsulfuration) in Pseudomonas fluorescens FW300-N2E2

Reactions and Genes	Cobalt chloride 0.6 mM
Aspartate transaminase (in reverse): oxaloacetate + L-glutamate→L-aspartate + 2-oxoglutarate (EC 2.6.1.1)
Pf6N2E2_1496	-0.7
Pf6N2E2_2127	-0.7
Pf6N2E2_2446	+0.1
Pf6N2E2_2632	-0.5
Pf6N2E2_3173	-0.2
Pf6N2E2_4288	+0.1
Pf6N2E2_4386	-0.5
Pf6N2E2_462	+0.3
Pf6N2E2_5300	-1.1
Pf6N2E2_5813	-0.2
Pf6N2E2_5890	+0.2
Pf6N2E2_5891	N.D.
Pf6N2E2_5895	+0.2
Pf6N2E2_5989	+2.0
Pf6N2E2_637	-0.1
Pf6N2E2_684	+0.2
Aspartate kinase: L-aspartate + ATP→L-aspartyl-4-phosphate + ADP (EC 2.7.2.4)
Pf6N2E2_5676	-0.9
Aspartate-semialdehyde dehydrogenase (in reverse): L-aspartyl-4-phosphate + NADPH + H+→L-aspartate 4-semialdehyde + NADP+ + phosphate (EC 1.2.1.11)
Pf6N2E2_2844	+0.5
Pf6N2E2_4232	+0.0
Pf6N2E2_67	N.D.
Pf6N2E2_68	+0.1
Homoserine dehydrogenase (in reverse): L-aspartate 4-semialdehyde + NAD(P)H + H+→L-homoserine + NAD(P)+ (EC 1.1.1.3)
Pf6N2E2_3143	N.D.
Pf6N2E2_4963	+0.1
Homoserine O-succinyltransferase: L-homoserine + succinyl-CoA→O-succinyl-L-homoserine + coenzyme A (EC 2.3.1.46)
No genes
Cystathionine gamma-synthase: O-succinyl-L-homoserine + L-cysteine→L-cystathionine + H+ + succinate (EC 2.5.1.48)
Pf6N2E2_4958	-0.0
Pf6N2E2_77	-0.2
Cysteine-S-conjugate beta-lyase: L-cystathionine + H2O→L-homocysteine + ammonium + pyruvate (EC 4.4.1.13)
No genes
5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase: L-homocysteine + 5-methyltetrahydropteroyl tri-L-glutamate→L-methionine + tetrahydropteroyl tri-L-glutamate (EC 2.1.1.14)
Pf6N2E2_5472	-0.1
Methionine synthase: L-homocysteine + a 5-methyltetrahydrofolate→L-methionine + a tetrahydrofolate (EC 2.1.1.13)
Pf6N2E2_2068	-0.7

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information