MetaCyc Pathway: cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation) in Pseudomonas fluorescens SBW25-INTG

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Reactions and Genes Glucose (C) and nitrate (N); with MOPS
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Uroporphyrinogen-III C-methyltransferase:
uroporphyrinogen-III + S-adenosyl-L-methionine→precorrin-1 + S-adenosyl-L-homocysteine + H+ (EC 2.1.1.107)
PFLU_RS18505 -3.2
PFLU_RS22590 -1.7
Uroporphyrinogen-III C-methyltransferase:
precorrin-1 + S-adenosyl-L-methionine→precorrin-2 + S-adenosyl-L-homocysteine (EC 2.1.1.107)
PFLU_RS18505 -3.2
PFLU_RS22590 -1.7
Precorrin-2 C(20)-methyltransferase:
precorrin-2 + S-adenosyl-L-methionine→precorrin-3A + S-adenosyl-L-homocysteine + H+ (EC 2.1.1.130)
PFLU_RS02985 -0.3
Precorrin-3B synthase:
precorrin-3A + NADH + dioxygen + H+→precorrin-3B + NAD+ + H2O (EC 1.14.13.83)
PFLU_RS02975 -0.5
Precorrin-3B C(17)-methyltransferase:
precorrin-3B + S-adenosyl-L-methionine→precorrin-4 + S-adenosyl-L-homocysteine + 2 H+ (EC 2.1.1.131)
PFLU_RS02990 -0.4
Precorrin-4 C(11)-methyltransferase:
precorrin-4 + S-adenosyl-L-methionine→precorrin-5 + S-adenosyl-L-homocysteine + H+ (EC 2.1.1.133)
PFLU_RS13015 -1.1
Precorrin-6A synthase (deacetylating):
precorrin-5 + S-adenosyl-L-methionine + H2O→precorrin-6A + acetate + S-adenosyl-L-homocysteine + 2 H+ (EC 2.1.1.152)
PFLU_RS11980 -0.6
Precorrin-6A reductase (in reverse):
precorrin-6A + NADPH + H+→precorrin-6B + NADP+ (EC 1.3.1.54)
PFLU_RS02965 -0.6
Precorrin-6Y C(5,15)-methyltransferase (decarboxylating):
precorrin-6B + H+ + S-adenosyl-L-methionine→precorrin-7 + S-adenosyl-L-homocysteine + CO2 (EC 2.1.1.132)
PFLU_RS02970 -0.5
Precorrin-6Y C(5,15)-methyltransferase (decarboxylating):
precorrin-7 + S-adenosyl-L-methionine→precorrin-8x + S-adenosyl-L-homocysteine + 2 H+ (EC 2.1.1.132)
PFLU_RS02970 -0.5
precorrin-8x + H+→hydrogenobyrinate (EC 5.4.99.61)
PFLU_RS02980 -0.9
Hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolyzing):
hydrogenobyrinate + 2 ATP + 2 L-glutamine + 2 H2O→hydrogenobyrinate a,c-diamide + 2 ADP + 2 L-glutamate + 2 H+ + 2 phosphate (EC 6.3.5.9)
PFLU_RS22025 -1.5
Cobaltochelatase:
hydrogenobyrinate a,c-diamide + ATP + Co2+ + H2O→cob(II)yrinate a,c-diamide + ADP + 3 H+ + phosphate (EC 6.6.1.2)
PFLU_RS13040 -0.5

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information