MetaCyc Pathway: NiFe(CO)(CN)2 cofactor biosynthesis in Klebsiella michiganensis M5al

Add experiment(s):


Reactions and Genes Fungal_growth_media_mCAFEs_v1 with Thallium(I) acetate 0.05 mg/mL
remove
[HypC dimer]-Fe-CO2 + a HypD [NiFe]-hydrogenase maturation protein→a [HypC/HypD dimer]-Fe-CO2 + a HypC [NiFe]-hydrogenase maturation protein
BWI76_RS22360 -0.2
Ni2+ + GTP + a HypB hydrogenase accessory protein + H2O→Ni-[HypB hydrogenase accessory protein] + GDP + H+ + phosphate
BWI76_RS25920 +0.0
carbamoyl phosphate + ATP + a HypE [NiFe]-hydrogenase maturation protein + H2O→a [HypE hydrogenase maturation protein]-S-carbamoyl-L-cysteine + AMP + diphosphate + H+ + phosphate
BWI76_RS22275 +0.3
a [HypE hydrogenase maturation protein]-S-carbamoyl-L-cysteine + ATP→a [HypE hydrogenase maturation protein]-S-cyanyl-L-cysteine + ADP + H+ + phosphate (EC 4.2.1.M8)
BWI76_RS22365 -0.1
a [HypC/HypD dimer]-Fe-CO2 + ATP + 2 a reduced one electron carrier→[HypC/HypD dimer]-Fe-CO + ADP + 2 an oxidized electron carrier + H+ + phosphate
BWI76_RS22360 -0.2
Ni-[HypB hydrogenase accessory protein] + a HypA hydrogenase accessory protein→Ni-[HypA hydrogenase accessory protein] + a HypB hydrogenase accessory protein
BWI76_RS22350 -0.3
[HypC/HypD dimer]-Fe-CO + 2 a [HypE hydrogenase maturation protein]-S-cyanyl-L-cysteine→[HypCDE]-Fe(CO)(CN)2
BWI76_RS22365 -0.1
[HypCDE]-Fe(CO)(CN)2 + a [NiFe]-hydrogenase large subunit→{[NiFe]-hydrogenase large subunit}-Fe(CO)(CN)2 + a HypCDE trimer
No genes
{[NiFe]-hydrogenase large subunit}-Fe(CO)(CN)2 + Ni-[HypA hydrogenase accessory protein]→[NiFe]-hydrogenase large subunit with unformed NiFe(CO)(CN)2 cofactor + a HypA hydrogenase accessory protein
BWI76_RS22345 +0.3
[NiFe]-hydrogenase large subunit with unformed NiFe(CO)(CN)2 cofactor + H2O→holo-[NiFe]-hydrogenase large subunit + an oligopeptide (EC 3.4.23.51)
BWI76_RS22300 +3.0

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information