MetaCyc Pathway: L-isoleucine biosynthesis I (from threonine) in Pseudomonas stutzeri RCH2

Reactions and Genes	L-Isoleucine (N)
Threonine ammonia-lyase: L-threonine→(Z)-2-aminobutenoate + H2O (EC 4.3.1.19)
Psest_3866	+0.1
1-aminocyclopropane-1-carboxylate deaminase: (Z)-2-aminobutenoate→2-iminobutanoate (spontaneous) (EC 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
Psest_2551	-0.4
Psest_3866	+0.1
2-iminobutanoate + H2O→2-oxobutanoate + ammonium (spontaneous) (EC 3.5.99.10; 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
Psest_2551	-0.4
Psest_3818	-0.3
Psest_3866	+0.1
Acetolactate synthase: 2-oxobutanoate + H+ + pyruvate→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)
Psest_1036	N.D.
Psest_1037	-1.2
Ketol-acid reductoisomerase (in reverse): (S)-2-aceto-2-hydroxybutanoate + NADPH + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NADP+ (EC 1.1.1.86)
Psest_1038	N.D.
Dihydroxy-acid dehydratase: (R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)
Psest_0234	N.D.
Branched-chain-amino-acid transaminase (in reverse): (3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
Psest_0450	-3.0

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information