MetaCyc Pathway: anandamide biosynthesis I in Pseudomonas stutzeri RCH2

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Reactions and Genes Doxycycline hyclate 0.0002 mg/ml
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Glycerol-3-phosphate O-acyltransferase:
arachidonoyl-CoA + sn-glycerol 3-phosphate→1-arachidinoyl-sn-glycerol 3-phosphate + coenzyme A (EC 2.3.1.15)
Psest_1608 +0.2
Psest_1686 -0.4
Psest_3678 -0.1
1-acylglycerol-3-phosphate O-acyltransferase:
1-arachidinoyl-sn-glycerol 3-phosphate + an acyl-CoA→a 1-arachidonoyl-2-acyl-sn-glycerol 3-phosphate + coenzyme A (EC 2.3.1.51)
Psest_1531 -1.2
Psest_3199 -0.2
Psest_0008 N.D.
Phosphatidate phosphatase:
a 1-arachidonoyl-2-acyl-sn-glycerol 3-phosphate + H2O→a 1-arachidonoyl-2-acyl-sn-glycerol + phosphate (EC 3.1.3.4)
No genes
Diacylglycerol cholinephosphotransferase:
a 1-arachidonoyl-2-acyl-sn-glycerol + CDP-choline→a 1-arachidonoyl-2-acyl-sn-glycero-3-phosphocholine + CMP + H+ (EC 2.7.8.2)
No genes
a 1-arachidonoyl-2-acyl-sn-glycero-3-phosphocholine + a phosphatidylethanolamine→an N-arachidonoyl-phosphatidylethanolamine + a 1-lysophosphatidylcholine + H+ (EC 2.3.1.M82)
No genes
an N-arachidonoyl-phosphatidylethanolamine + H2O→anandamide + a phosphatidate + H+ (EC 3.1.4.54)
No genes
Phospholipase A(2):
an N-arachidonoyl-phosphatidylethanolamine + H2O→an N-arachidonoyl-2-lysophosphatidylethanolamine + a fatty acid + H+ (EC 3.1.1.4)
No genes
Phospholipase A(1):
an N-arachidonoyl-phosphatidylethanolamine + H2O→an N-arachidonoyl-1-lysophosphatidylethanolamine + a fatty acid + H+ (EC 3.1.1.32)
No genes
Alkylglycerophosphoethanolamine phosphodiesterase:
an N-arachidonoyl-2-lysophosphatidylethanolamine + H2O→anandamide + a 2-lysophosphatidate + H+ (EC 3.1.4.39)
No genes
Phospholipase A(2):
an N-arachidonoyl-2-lysophosphatidylethanolamine + H2O→sn-glycero-3-phospho-N-(arachidonoyl)ethanolamine + a fatty acid + H+ (EC 3.1.1.4)
No genes
Phospholipase A(2):
an N-arachidonoyl-1-lysophosphatidylethanolamine + H2O→sn-glycero-3-phospho-N-(arachidonoyl)ethanolamine + a fatty acid + H+ (EC 3.1.1.4)
No genes
Glycerophosphodiester phosphodiesterase:
sn-glycero-3-phospho-N-(arachidonoyl)ethanolamine + H2O→anandamide + sn-glycerol 3-phosphate + H+ (EC 3.1.4.46)
Psest_1664 -0.2
Psest_0228 +0.0

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information