MetaCyc Pathway: L-glutamate and L-glutamine biosynthesis in Pseudomonas stutzeri RCH2

Reactions and Genes	L-Citrulline (N)
Glutamate dehydrogenase (NAD(P)(+)) (in reverse): 2-oxoglutarate + ammonium + NAD(P)H + H+→L-glutamate + NAD(P)+ + H2O (EC 1.4.1.3)
No genes
Glutamate synthase (ferredoxin) (in reverse): 2-oxoglutarate + L-glutamine + 2 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→2 L-glutamate + 2 an oxidized ferredoxin [iron-sulfur] cluster (EC 1.4.7.1)
No genes
Glutamate synthase (NADH) (in reverse): 2-oxoglutarate + L-glutamine + NADH + H+→2 L-glutamate + NAD+ (EC 1.4.1.14)
Psest_3710	-3.3
Psest_3711	-3.2
Glutamate synthase (NADPH): L-glutamine + H2O→L-glutamate + ammonia + H+ (EC 1.4.1.13; 1.4.1.14; 1.4.7.1; 2.6.1.85; 3.5.1.2; 3.5.1.38; 4.1.3.27; 4.3.2.10; 4.3.3.6; 6.3.4.2; 6.3.5.13; 6.3.5.2; 6.3.5.4; 6.3.5.5; 6.3.5.6; 6.3.5.7)
Psest_1168	N.D.
Psest_0117	-0.3
Psest_1287	N.D.
Psest_2326	+0.1
Psest_2644	N.D.
Psest_2824	N.D.
Psest_2930	+0.2
Psest_3135	N.D.
Psest_3175	N.D.
Psest_3328	N.D.
Psest_3329	N.D.
Psest_3330	N.D.
Psest_3555	-0.3
Psest_3649	-3.8
Psest_3650	-3.3
Psest_3710	-3.3
Psest_3711	-3.2
Psest_4081	+0.2
Psest_0967	N.D.
Psest_0968	N.D.
Glutamate--ammonia ligase: L-glutamate + ammonium + ATP→L-glutamine + ADP + H+ + phosphate (EC 6.3.1.2)
Psest_2367	+0.0
Psest_3928	N.D.
Psest_4286	+0.2
Psest_4288	-2.7
Glutamate dehydrogenase (NADP(+)) (in reverse): 2-oxoglutarate + ammonium + NADPH + H+→L-glutamate + NADP+ + H2O (EC 1.4.1.4)
Psest_3438	-0.1
Psest_3710	-3.3
Glutamate synthase (NADPH) (in reverse): 2-oxoglutarate + ammonia + NADPH + 2 H+→L-glutamate + NADP+ + H2O (EC 1.4.1.13; 1.4.1.14)
Psest_1168	N.D.
Psest_0117	-0.3
Psest_2644	N.D.
Psest_3135	N.D.
Psest_3175	N.D.
Psest_3555	-0.3
Psest_3710	-3.3
Psest_3711	-3.2

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information