MetaCyc Pathway: superpathway of L-methionine biosynthesis (transsulfuration) in Pseudomonas fluorescens SBW25-INTG

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Reactions and Genes Sucrose (C) and Glycine betaine (N); with TAPS; with PEG
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Aspartate transaminase (in reverse):
oxaloacetate + L-glutamate→L-aspartate + 2-oxoglutarate (EC 2.6.1.1)
PFLU_RS08510 +0.0
PFLU_RS09125 +0.0
PFLU_RS10980 -0.1
PFLU_RS13455 +0.0
PFLU_RS15480 +0.1
PFLU_RS16880 -0.1
PFLU_RS17055 -0.0
PFLU_RS17810 -0.1
PFLU_RS19680 +0.1
PFLU_RS21080 -0.1
PFLU_RS21355 -0.0
PFLU_RS21880 -0.1
PFLU_RS22640 +2.4
PFLU_RS22895 +0.1
PFLU_RS24775 +0.0
PFLU_RS29405 +0.1
PFLU_RS29850 +0.1
Aspartate kinase:
L-aspartate + ATP→L-aspartyl-4-phosphate + ADP (EC 2.7.2.4)
PFLU_RS23265 +0.1
Aspartate-semialdehyde dehydrogenase (in reverse):
L-aspartyl-4-phosphate + NADPH + H+→L-aspartate 4-semialdehyde + NADP+ + phosphate (EC 1.2.1.11)
PFLU_RS06680 -0.2
PFLU_RS20555 -0.2
PFLU_RS20560 N.D.
Homoserine dehydrogenase (in reverse):
L-aspartate 4-semialdehyde + NAD(P)H + H+→L-homoserine + NAD(P)+ (EC 1.1.1.3)
PFLU_RS09890 -0.0
PFLU_RS24550 N.D.
Homoserine O-succinyltransferase:
L-homoserine + succinyl-CoA→O-succinyl-L-homoserine + coenzyme A (EC 2.3.1.46)
No genes
Cystathionine gamma-synthase:
O-succinyl-L-homoserine + L-cysteine→L-cystathionine + H+ + succinate (EC 2.5.1.48)
PFLU_RS02250 +0.1
PFLU_RS16625 -0.1
PFLU_RS20510 -3.0
Cysteine-S-conjugate beta-lyase:
L-cystathionine + H2O→L-homocysteine + ammonium + pyruvate (EC 4.4.1.13)
No genes
5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase:
L-homocysteine + 5-methyltetrahydropteroyl tri-L-glutamate→L-methionine + tetrahydropteroyl tri-L-glutamate (EC 2.1.1.14)
PFLU_RS17405 -0.0
Methionine synthase:
L-homocysteine + a 5-methyltetrahydrofolate→L-methionine + a tetrahydrofolate (EC 2.1.1.13)
PFLU_RS13000 -0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information