MetaCyc Pathway: L-asparagine biosynthesis III (tRNA-dependent) in Pseudomonas stutzeri RCH2

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Reactions and Genes L-Glutamine (C)
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Glutamate synthase (NADPH):
L-glutamine + H2O→ammonia + L-glutamate + H+ (EC 1.4.1.13; 1.4.1.14; 1.4.7.1; 2.6.1.85; 3.5.1.2; 3.5.1.38; 4.1.3.27; 4.3.2.10; 4.3.3.6; 6.3.4.2; 6.3.5.13; 6.3.5.2; 6.3.5.4; 6.3.5.5; 6.3.5.6; 6.3.5.7)
Psest_1168 N.D.
Psest_0117 -0.2
Psest_1287 N.D.
Psest_2326 +0.0
Psest_2644 N.D.
Psest_2824 N.D.
Psest_2930 +0.0
Psest_3135 N.D.
Psest_3175 N.D.
Psest_3328 N.D.
Psest_3329 N.D.
Psest_3330 N.D.
Psest_3555 -0.2
Psest_3649 -3.5
Psest_3650 -6.4
Psest_3710 -0.1
Psest_3711 -0.1
Psest_4081 -4.5
Psest_0967 N.D.
Psest_0968 N.D.
Aspartate--tRNA(Asn) ligase:
L-aspartate + tRNAAsn + ATP→an L-aspartyl-[tRNAAsn] + AMP + diphosphate (EC 6.1.1.23)
Psest_1493 N.D.
Asparaginyl-tRNA synthase (glutamine-hydrolyzing):
an L-aspartyl-[tRNAAsn] + ATP→a 4-phosphooxy-L-aspartyl-[tRNAAsn] + ADP (EC 6.3.5.6)
Psest_3328 N.D.
Psest_3329 N.D.
Psest_3330 N.D.
Asparaginyl-tRNA synthase (glutamine-hydrolyzing):
a 4-phosphooxy-L-aspartyl-[tRNAAsn] + ammonia→an L-asparaginyl-[tRNAAsn] + phosphate (EC 6.3.5.6)
Psest_3328 N.D.
Psest_3329 N.D.
Psest_3330 N.D.

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information