MetaCyc Pathway: L-glutamate degradation V (via hydroxyglutarate) in Pantoea sp. MT58

Reactions and Genes	Ying_sugar7 rep B; time point 3
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Glutamate dehydrogenase: L-glutamate + NAD⁺ + H₂O→2-oxoglutarate + ammonium + NADH + H⁺ (EC 1.4.1.2)
IAI47_20110	-0.0
2-oxoglutarate + NADH + H⁺→(R)-2-hydroxyglutarate + NAD⁺ (EC 1.1.1.399)
IAI47_03725	-4.8
Glutaconate CoA-transferase: (R)-2-hydroxyglutarate + acetyl-CoA→(R)-2-hydroxyglutaryl-CoA + acetate (EC 2.8.3.12)
No genes
(R)-2-hydroxyglutaryl-CoA→(E)-glutaconyl-CoA + H₂O (EC 4.2.1.167)
No genes
(E)-glutaconyl-CoA_[in] + Na⁺_[in] + H⁺_[in]→crotonyl-CoA_[in] + CO₂_[in] + Na⁺_[out] (EC 7.2.4.5)
No genes
crotonyl-CoA + H₂O→(S)-3-hydroxybutanoyl-CoA (EC 4.2.1.150)
IAI47_06130	-0.1
IAI47_09300	-1.7
IAI47_18230	-0.0
crotonyl-CoA_[cytosol] + 2 NADH_[cytosol] + 2 an oxidized ferredoxin [iron-sulfur] cluster_[cytosol]→butanoyl-CoA_[cytosol] + 2 NAD⁺_[cytosol] + 2 a reduced ferredoxin [iron-sulfur] cluster_[cytosol] (EC 1.3.1.109)
No genes
Propionate CoA-transferase: butanoyl-CoA + acetate→acetyl-CoA + butanoate (EC 2.8.3.1; 2.8.3.8)
No genes
3-hydroxyacyl-CoA dehydrogenase: (S)-3-hydroxybutanoyl-CoA + NAD⁺→acetoacetyl-CoA + NADH + H⁺ (EC 1.1.1.35)
IAI47_06130	-0.1
IAI47_18230	-0.0
Acetyl-CoA C-acyltransferase (in reverse): acetoacetyl-CoA + coenzyme A→2 acetyl-CoA (EC 2.3.1.16; 2.3.1.9)
IAI47_06125	+0.2
IAI47_18235	-0.1

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Pathway details at MetaCyc
Fitness data for 7 genes
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All MetaCyc pathways for Pantoea sp. MT58

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information