MetaCyc Pathway: acetyl-CoA fermentation to butanoate in Klebsiella michiganensis M5al

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Reactions and Genes 2-Deoxy-D-ribonic lithium salt 10 mM (C)
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Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
BWI76_RS01360 +0.1
BWI76_RS13135 +0.0
BWI76_RS16135 +0.3
BWI76_RS20460 +0.0
BWI76_RS23445 +0.0
BWI76_RS23710 -2.9
Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
BWI76_RS03560 +0.6
BWI76_RS20240 N.D.
BWI76_RS20805 +0.6
BWI76_RS23150 -0.6
Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
BWI76_RS18090 -0.6
BWI76_RS20235 N.D.
BWI76_RS20815 -0.3
BWI76_RS20820 +0.2
BWI76_RS23200 -0.2
Acetoacetyl-CoA reductase (in reverse):
acetoacetyl-CoA + NADPH + H+→(3R)-3-hydroxybutanoyl-CoA + NADP+ (EC 1.1.1.36)
No genes
3-hydroxybutyryl-CoA dehydratase:
(3R)-3-hydroxybutanoyl-CoA→crotonyl-CoA + H2O (EC 4.2.1.55)
BWI76_RS13085 +0.4
crotonyl-CoA[cytosol] + 2 NADH[cytosol] + 2 an oxidized ferredoxin [iron-sulfur] cluster[cytosol]→butanoyl-CoA[cytosol] + 2 NAD+[cytosol] + 2 a reduced ferredoxin [iron-sulfur] cluster[cytosol] (EC 1.3.1.109)
No genes
Propionate CoA-transferase:
acetate + butanoyl-CoA→acetyl-CoA + butanoate (EC 2.8.3.1; 2.8.3.8)
BWI76_RS23700 -3.5

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information