MetaCyc Pathway: L-glutamate degradation V (via hydroxyglutarate) in Klebsiella michiganensis M5al

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Reactions and Genes 2-Deoxy-D-ribonic lithium salt 10 mM (C)
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Glutamate dehydrogenase:
L-glutamate + NAD+ + H2O→2-oxoglutarate + ammonium + NADH + H+ (EC 1.4.1.2)
BWI76_RS13195 +0.2
2-oxoglutarate + NADH + H+→(R)-2-hydroxyglutarate + NAD+ (EC 1.1.1.399)
BWI76_RS23920 -6.2
Glutaconate CoA-transferase:
(R)-2-hydroxyglutarate + acetyl-CoA→(R)-2-hydroxyglutaryl-CoA + acetate (EC 2.8.3.12)
No genes
(R)-2-hydroxyglutaryl-CoA→(E)-glutaconyl-CoA + H2O (EC 4.2.1.167)
No genes
(E)-glutaconyl-CoA[in] + Na+[in] + H+[in]→crotonyl-CoA[in] + CO2[in] + Na+[out] (EC 7.2.4.5)
No genes
crotonyl-CoA + H2O→(S)-3-hydroxybutanoyl-CoA (EC 4.2.1.150)
BWI76_RS01365 -0.2
BWI76_RS01370 N.D.
BWI76_RS20455 +0.1
crotonyl-CoA[cytosol] + 2 NADH[cytosol] + 2 an oxidized ferredoxin [iron-sulfur] cluster[cytosol]→butanoyl-CoA[cytosol] + 2 NAD+[cytosol] + 2 a reduced ferredoxin [iron-sulfur] cluster[cytosol] (EC 1.3.1.109)
No genes
Propionate CoA-transferase:
butanoyl-CoA + acetate→acetyl-CoA + butanoate (EC 2.8.3.1; 2.8.3.8)
BWI76_RS23700 -3.5
3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxybutanoyl-CoA + NAD+→acetoacetyl-CoA + NADH + H+ (EC 1.1.1.35)
BWI76_RS01365 -0.2
BWI76_RS01370 N.D.
BWI76_RS20455 +0.1
Acetyl-CoA C-acyltransferase (in reverse):
acetoacetyl-CoA + coenzyme A→2 acetyl-CoA (EC 2.3.1.16; 2.3.1.9)
BWI76_RS01360 +0.1
BWI76_RS13135 +0.0
BWI76_RS16135 +0.3
BWI76_RS20460 +0.0
BWI76_RS23445 +0.0
BWI76_RS23710 -2.9

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information