MetaCyc Pathway: L-isoleucine biosynthesis V in Klebsiella michiganensis M5al

Add experiment(s):


Reactions and Genes 2'-Deoxycytidine 10 mM (C)
remove
Acetate--CoA ligase:
2-methylbutanoate + ATP + coenzyme A→(2S)-2-methylbutanoyl-CoA + AMP + diphosphate (EC 6.2.1.1)
BWI76_RS02095 -1.6
3-methyl-2-oxobutanoate dehydrogenase (ferredoxin) (in reverse):
(2S)-2-methylbutanoyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→(3S)-3-methyl-2-oxopentanoate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster (EC 1.2.7.7)
No genes
Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
BWI76_RS00970 -0.2
BWI76_RS10365 -0.0
BWI76_RS24235 +0.0

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information