MetaCyc Pathway: TCA cycle I (prokaryotic) in Pseudomonas fluorescens SBW25-INTG

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Reactions and Genes malate (C) and Ammonium chloride (N); with TAPS
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2-oxoglutarate + coenzyme A + NAD+→succinyl-CoA + CO2 + NADH (EC 1.2.1.105)
PFLU_RS19110 +0.0
Succinate--CoA ligase (ADP-forming) (in reverse):
succinyl-CoA + ADP + phosphate→succinate + ATP + coenzyme A (EC 6.2.1.5)
PFLU_RS08970 N.D.
PFLU_RS08975 N.D.
Succinate dehydrogenase (ubiquinone):
succinate[in] + an electron-transfer quinone→fumarate[in] + an electron-transfer quinol (EC 1.3.5.1)
PFLU_RS08935 N.D.
PFLU_RS08940 N.D.
PFLU_RS08945 N.D.
PFLU_RS08950 N.D.
Fumarate hydratase (in reverse):
fumarate + H2O→(S)-malate (EC 4.2.1.2)
PFLU_RS04360 +0.1
PFLU_RS21170 -0.0
PFLU_RS24270 N.D.
Malate dehydrogenase:
(S)-malate + NAD+→oxaloacetate + NADH + H+ (EC 1.1.1.37; 1.1.1.38)
No genes
(S)-malate[in] + an electron-transfer quinone→oxaloacetate[in] + an electron-transfer quinol (EC 1.1.5.4)
PFLU_RS04510 N.D.
PFLU_RS07940 -2.1
Citrate (Si)-synthase:
oxaloacetate + acetyl-CoA + H2O→citrate + coenzyme A + H+ (EC 2.3.3.1; 2.3.3.16; 2.3.3.3)
PFLU_RS08930 N.D.
Aconitate hydratase:
citrate→cis-aconitate + H2O (EC 4.2.1.3)
PFLU_RS07595 -0.0
PFLU_RS17020 N.D.
PFLU_RS22680 -0.3
Aconitate hydratase:
cis-aconitate + H2O→D-threo-isocitrate (EC 4.2.1.3)
PFLU_RS07595 -0.0
PFLU_RS17020 N.D.
PFLU_RS22680 -0.3
Isocitrate dehydrogenase (NADP(+)):
D-threo-isocitrate + NADP+→2-oxoglutarate + CO2 + NADPH (EC 1.1.1.42)
PFLU_RS18565 +0.0
PFLU_RS18570 N.D.

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information