MetaCyc Pathway: 2-methyl-branched fatty acid β-oxidation in Klebsiella michiganensis M5al

Add experiment(s):


Reactions and Genes 2-Deoxy-D-ribonic lithium salt 10 mM (C)
remove
Butyrate--CoA ligase:
a short-chain 2-methyl-branched fatty acid + ATP + coenzyme A→a short-chain 2-methyl fatty acyl-CoA + AMP + diphosphate (EC 6.2.1.2)
No genes
a long-chain 2-methyl fatty acyl-CoA + an oxidized electron-transfer flavoprotein + H+→a long-chain (2E)-2-methyl-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.8)
No genes
Butyrate--CoA ligase:
a medium-chain 2-methyl-branched fatty acid + ATP + coenzyme A→a medium-chain 2-methyl fatty acyl-CoA + AMP + diphosphate (EC 6.2.1.2)
No genes
a short-chain 2-methyl fatty acyl-CoA + an oxidized electron-transfer flavoprotein + H+→a short-chain (2E)-2-methyl-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.5)
No genes
a medium-chain 2-methyl fatty acyl-CoA + an oxidized electron-transfer flavoprotein + H+→a medium-chain (2E)-2-methyl-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.7)
BWI76_RS02590 -0.1
Enoyl-CoA hydratase (in reverse):
a long-chain (2E)-2-methyl-2-enoyl-CoA + H2O→a long-chain (3S)-3-hydroxy-2-methylacyl-CoA (EC 4.2.1.17)
BWI76_RS01365 -0.0
BWI76_RS01370 N.D.
BWI76_RS13115 N.D.
BWI76_RS13120 N.D.
BWI76_RS20455 +0.5
Enoyl-CoA hydratase (in reverse):
a short-chain (2E)-2-methyl-2-enoyl-CoA + H2O→a short-chain (3S)-3-hydroxy-2-methylacyl-CoA (EC 4.2.1.17)
BWI76_RS01365 -0.0
BWI76_RS01370 N.D.
BWI76_RS13115 N.D.
BWI76_RS13120 N.D.
BWI76_RS20455 +0.5
Enoyl-CoA hydratase (in reverse):
a medium-chain (2E)-2-methyl-2-enoyl-CoA + H2O→a medium-chain (3S)-3-hydroxy-2-methylacyl-CoA (EC 4.2.1.17)
BWI76_RS01365 -0.0
BWI76_RS01370 N.D.
BWI76_RS13115 N.D.
BWI76_RS13120 N.D.
BWI76_RS20455 +0.5
3-hydroxyacyl-CoA dehydrogenase:
a long-chain (3S)-3-hydroxy-2-methylacyl-CoA + NAD+→a long-chain 2-methyl-3-oxoacyl-CoA + NADH + H+ (EC 1.1.1.35)
BWI76_RS01365 -0.0
BWI76_RS01370 N.D.
BWI76_RS20455 +0.5
3-hydroxy-2-methylbutyryl-CoA dehydrogenase:
a short-chain (3S)-3-hydroxy-2-methylacyl-CoA + NAD+→a short-chain 2-methyl-3-oxoacyl-CoA + NADH + H+ (EC 1.1.1.178)
No genes
3-hydroxyacyl-CoA dehydrogenase:
a medium-chain (3S)-3-hydroxy-2-methylacyl-CoA + NAD+→a medium-chain 2-methyl-3-oxoacyl-CoA + NADH + H+ (EC 1.1.1.35)
BWI76_RS01365 -0.0
BWI76_RS01370 N.D.
BWI76_RS20455 +0.5
Acetyl-CoA C-acyltransferase (in reverse):
a long-chain 2-methyl-3-oxoacyl-CoA + coenzyme A→a long-chain 2-methyl fatty acyl-CoA + propanoyl-CoA (EC 2.3.1.16)
BWI76_RS01360 +0.1
BWI76_RS13135 +0.6
BWI76_RS16135 +0.0
BWI76_RS20460 -0.2
BWI76_RS23445 -0.2
BWI76_RS23710 -3.8
Acetyl-CoA C-acyltransferase (in reverse):
a short-chain 2-methyl-3-oxoacyl-CoA + coenzyme A→a short-chain 2-methyl fatty acyl-CoA + propanoyl-CoA (EC 2.3.1.16)
BWI76_RS01360 +0.1
BWI76_RS13135 +0.6
BWI76_RS16135 +0.0
BWI76_RS20460 -0.2
BWI76_RS23445 -0.2
BWI76_RS23710 -3.8
Acetyl-CoA C-acyltransferase (in reverse):
a medium-chain 2-methyl-3-oxoacyl-CoA + coenzyme A→a medium-chain 2-methyl fatty acyl-CoA + propanoyl-CoA (EC 2.3.1.16)
BWI76_RS01360 +0.1
BWI76_RS13135 +0.6
BWI76_RS16135 +0.0
BWI76_RS20460 -0.2
BWI76_RS23445 -0.2
BWI76_RS23710 -3.8

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information