| Reactions and Genes | 2-Deoxy-D-ribonic lithium salt 10 mM (C) |
|---|
| remove |
sn-glycerol-1-phosphate dehydrogenase (in reverse):
glycerone phosphate + NAD(P)H + H+→sn-glycerol 1-phosphate + NAD(P)+ (EC 1.1.1.261) |
| No genes |
Carbon-monoxide dehydrogenase (ferredoxin) (in reverse):
CO2 + 2 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→carbon monoxide + 2 an oxidized ferredoxin [iron-sulfur] cluster + H2O (EC 1.2.7.4; 2.3.3.M4; 2.3.3.M5) |
| No genes |
| CO2 + NADPH→formate + NADP+ (EC 1.17.1.10) |
| No genes |
| CO2 + a methanofuran + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→a formylmethanofuran + 2 an oxidized ferredoxin [iron-sulfur] cluster + H2O (EC 1.2.7.12) |
| No genes |
Carbonate dehydratase (in reverse):
CO2 + H2O→hydrogen carbonate + H+ (EC 4.2.1.1) |
| BWI76_RS04960 | -0.6 |
| BWI76_RS16635 | -0.0 |
| BWI76_RS25560 | -0.0 |
Formylmethanofuran--tetrahydromethanopterin N-formyltransferase:
a formylmethanofuran + tetrahydromethanopterin + H+→5-formyl-tetrahydromethanopterin + a methanofuran (EC 2.3.1.101) |
| No genes |
Formate--tetrahydrofolate ligase:
formate + ATP + a tetrahydrofolate→an N10-formyltetrahydrofolate + ADP + phosphate (EC 6.3.4.3) |
| No genes |
Methenyltetrahydromethanopterin cyclohydrolase (in reverse):
5-formyl-tetrahydromethanopterin + H+→5,10-methenyltetrahydromethanopterin + H2O (EC 3.5.4.27) |
| No genes |
Methenyltetrahydrofolate cyclohydrolase (in reverse):
an N10-formyltetrahydrofolate + H+→a 5,10-methenyltetrahydrofolate + H2O (EC 3.5.4.9) |
| BWI76_RS07100 | N.D. |
5,10-methenyltetrahydromethanopterin hydrogenase:
5,10-methenyltetrahydromethanopterin + H2→5,10-methylene-tetrahydromethanopterin + H+ (EC 1.12.98.2) |
| No genes |
Methylenetetrahydrofolate dehydrogenase (NADP(+)) (in reverse):
a 5,10-methenyltetrahydrofolate + NADPH→a 5,10-methylenetetrahydrofolate + NADP+ (EC 1.5.1.5) |
| BWI76_RS07100 | N.D. |
| 5,10-methylene-tetrahydromethanopterin + a reduced factor 420→5-methyltetrahydromethanopterin + an oxidized factor 420 (EC 1.5.98.2) |
| No genes |
| a 5,10-methylenetetrahydrofolate + 2 NADH + 2 an oxidized ferredoxin [iron-sulfur] cluster→a 5-methyltetrahydrofolate + 2 NAD+ + 2 a reduced ferredoxin [iron-sulfur] cluster (EC 1.5.1.M4) |
| No genes |
| a 5,10-methylenetetrahydrofolate + NADH + H+→a 5-methyltetrahydrofolate + NAD+ (EC 1.5.1.54) |
| BWI76_RS00805 | -7.3 |
Methylenetetrahydrofolate reductase (ferredoxin) (in reverse):
a 5,10-methylenetetrahydrofolate + 2 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→a 5-methyltetrahydrofolate + 2 an oxidized ferredoxin [iron-sulfur] cluster (EC 1.5.7.1) |
| No genes |
| a 5-methyltetrahydrofolate + a [Co(I) corrinoid Fe-S protein] + H+→a [methyl-Co(III) corrinoid Fe-S protein] + a tetrahydrofolate (EC 2.1.1.258) |
| No genes |
| CO2 + 5-methyltetrahydromethanopterin + coenzyme A + 2 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→tetrahydromethanopterin + acetyl-CoA + 2 an oxidized ferredoxin [iron-sulfur] cluster + H2O (EC 2.3.3.M4) |
| No genes |
CO-methylating acetyl-CoA synthase (in reverse):
carbon monoxide + a [methyl-Co(III) corrinoid Fe-S protein] + coenzyme A→acetyl-CoA + a [Co(I) corrinoid Fe-S protein] + H+ (EC 2.3.1.169; 2.3.3.M4; 2.3.3.M5) |
| No genes |
Pyruvate synthase (in reverse):
acetyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→pyruvate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster (EC 1.2.7.1) |
| BWI76_RS12730 | -0.1 |
| BWI76_RS18805 | +0.0 |
Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9) |
| BWI76_RS01360 | +0.1 |
| BWI76_RS13135 | +0.6 |
| BWI76_RS16135 | +0.0 |
| BWI76_RS20460 | -0.2 |
| BWI76_RS23445 | -0.2 |
| BWI76_RS23710 | -3.8 |
Alanine transaminase (in reverse):
pyruvate + L-glutamate→L-alanine + 2-oxoglutarate (EC 2.6.1.2) |
| BWI76_RS20205 | +0.2 |
| BWI76_RS20495 | -0.1 |
Pyruvate, water dikinase:
pyruvate + ATP + H2O→phosphoenolpyruvate + AMP + 2 H+ + phosphate (EC 2.7.9.2) |
| BWI76_RS17065 | +0.1 |
Pyruvate carboxylase:
hydrogen carbonate + pyruvate + ATP→oxaloacetate + ADP + H+ + phosphate (EC 6.4.1.1) |
| No genes |
Phosphopyruvate hydratase (in reverse):
phosphoenolpyruvate + H2O→2-phospho-D-glycerate (EC 4.2.1.11) |
| BWI76_RS22790 | N.D. |
Hydroxymethylglutaryl-CoA synthase:
acetoacetyl-CoA + acetyl-CoA + H2O→(S)-3-hydroxy-3-methylglutaryl-CoA + coenzyme A + H+ (EC 2.3.3.10) |
| No genes |
Phosphoenolpyruvate carboxylase (in reverse):
hydrogen carbonate + phosphoenolpyruvate→oxaloacetate + phosphate (EC 4.1.1.31) |
| BWI76_RS00835 | -3.8 |
Hydroxymethylglutaryl-CoA reductase (NADPH) (in reverse):
(S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+→(R)-mevalonate + coenzyme A + 2 NADP+ (EC 1.1.1.34) |
| No genes |
| 2-phospho-D-glycerate→3-phospho-D-glycerate (EC 5.4.2.12) |
| BWI76_RS04200 | +0.0 |
| BWI76_RS08605 | -0.1 |
| BWI76_RS13025 | -0.0 |
| BWI76_RS27230 | -0.1 |
Aspartate transaminase (in reverse):
oxaloacetate + L-glutamate→L-aspartate + 2-oxoglutarate (EC 2.6.1.1) |
| BWI76_RS03690 | -0.4 |
| BWI76_RS06660 | +0.2 |
| BWI76_RS06750 | -0.2 |
| BWI76_RS07560 | +0.1 |
| BWI76_RS07570 | -0.3 |
| BWI76_RS08675 | +0.0 |
| BWI76_RS09870 | -0.5 |
| BWI76_RS10245 | +0.6 |
| BWI76_RS12985 | +0.8 |
| BWI76_RS13400 | -0.8 |
| BWI76_RS20205 | +0.2 |
| BWI76_RS22035 | -0.0 |
| BWI76_RS24235 | +0.3 |
Malate dehydrogenase (in reverse):
oxaloacetate + NADH + H+→(S)-malate + NAD+ (EC 1.1.1.37; 1.1.1.38) |
| BWI76_RS13830 | -0.5 |
| BWI76_RS16775 | +0.2 |
| BWI76_RS25420 | -0.9 |
Glyceraldehyde-3-phosphate dehydrogenase (ferredoxin) (in reverse):
3-phospho-D-glycerate + 3 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→D-glyceraldehyde 3-phosphate + 2 an oxidized ferredoxin [iron-sulfur] cluster + H2O (EC 1.2.7.6) |
| No genes |
Fumarate hydratase:
(S)-malate→fumarate + H2O (EC 4.2.1.2) |
| BWI76_RS08450 | -0.1 |
| BWI76_RS16115 | -0.3 |
| BWI76_RS16270 | +0.1 |
| BWI76_RS16275 | -0.4 |
| BWI76_RS16955 | -0.4 |
Mevalonate kinase:
(R)-mevalonate + ATP→(R)-5-phosphomevalonate + ADP + H+ (EC 2.7.1.36) |
| No genes |
Phosphomevalonate kinase:
(R)-5-phosphomevalonate + ATP→(R)-mevalonate diphosphate + ADP (EC 2.7.4.2) |
| No genes |
Succinate dehydrogenase (ubiquinone):
fumarate[in] + a menaquinol→succinate[in] + a menaquinone (EC 1.3.5.1) |
| BWI76_RS02440 | +0.3 |
| BWI76_RS02445 | -0.2 |
| BWI76_RS02450 | +0.3 |
| BWI76_RS02455 | -0.0 |
Triose-phosphate isomerase:
D-glyceraldehyde 3-phosphate→glycerone phosphate (EC 5.3.1.1) |
| BWI76_RS03100 | -0.3 |
| BWI76_RS27465 | N.D. |
Diphosphomevalonate decarboxylase:
(R)-mevalonate diphosphate + ATP→3-methylbut-3-en-1-yl diphosphate + CO2 + ADP + phosphate (EC 4.1.1.33) |
| No genes |
Fructose-bisphosphate aldolase (in reverse):
glycerone phosphate + D-glyceraldehyde 3-phosphate→β-D-fructofuranose 1,6-bisphosphate (EC 4.1.2.13) |
| BWI76_RS00545 | -0.0 |
| BWI76_RS14880 | -0.2 |
| BWI76_RS19370 | +0.5 |
| BWI76_RS23975 | N.D. |
| BWI76_RS27560 | -0.7 |
Succinate--CoA ligase (ADP-forming):
succinate + ATP + coenzyme A→succinyl-CoA + ADP + phosphate (EC 6.2.1.5) |
| BWI76_RS08425 | -3.2 |
| BWI76_RS08430 | -2.0 |
2-oxoglutarate synthase (in reverse):
succinyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→2-oxoglutarate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster (EC 1.2.7.3) |
| No genes |
Isopentenyl-diphosphate Delta-isomerase:
3-methylbut-3-en-1-yl diphosphate→prenyl diphosphate (EC 5.3.3.2) |
| BWI76_RS23790 | N.D. |
ADP-specific phosphofructokinase (in reverse):
β-D-fructofuranose 1,6-bisphosphate + AMP + H+→β-D-fructofuranose 6-phosphate + ADP (EC 2.7.1.146) |
| No genes |
Dimethylallyltranstransferase:
prenyl diphosphate + 3-methylbut-3-en-1-yl diphosphate→geranyl diphosphate + diphosphate (EC 2.5.1.1) |
| BWI76_RS06385 | N.D. |
Glucose-6-phosphate isomerase (in reverse):
β-D-fructofuranose 6-phosphate→α-D-glucose 6-phosphate (EC 5.3.1.9) |
| BWI76_RS01800 | +0.6 |
Geranyltranstransferase:
geranyl diphosphate + 3-methylbut-3-en-1-yl diphosphate→(2E,6E)-farnesyl diphosphate + diphosphate (EC 2.5.1.10) |
| BWI76_RS06385 | N.D. |
Farnesyltranstransferase:
(2E,6E)-farnesyl diphosphate + 3-methylbut-3-en-1-yl diphosphate→geranylgeranyl diphosphate + diphosphate (EC 2.5.1.29) |
| BWI76_RS06385 | N.D. |
Phosphoglycerol geranylgeranyltransferase:
geranylgeranyl diphosphate + sn-glycerol 1-phosphate→sn-3-(O-geranylgeranyl)glycerol 1-phosphate + diphosphate (EC 2.5.1.41) |
| No genes |
Geranylgeranylglycerol-phosphate geranylgeranyltransferase:
geranylgeranyl diphosphate + sn-3-(O-geranylgeranyl)glycerol 1-phosphate→2,3-bis-O-geranylgeranyl-sn-glycerol 1-phosphate + diphosphate (EC 2.5.1.42) |
| No genes |
| 2,3-bis-O-geranylgeranyl-sn-glycerol 1-phosphate + 8 NAD(P)H + 8 H+→2,3-bis-O-phytanyl-sn-glycerol 1-phosphate + 8 NAD(P)+ (EC 1.3.1.101) |
| No genes |
| 2,3-bis-O-geranylgeranyl-sn-glycerol 1-phosphate + 16 H+ + 16 a reduced ferredoxin [iron-sulfur] cluster→2,3-bis-O-phytanyl-sn-glycerol 1-phosphate + 16 an oxidized ferredoxin [iron-sulfur] cluster (EC 1.3.7.11) |
| No genes |
CDP-archaeol synthase:
2,3-bis-O-geranylgeranyl-sn-glycerol 1-phosphate + CTP + H+→CDP-2,3-bis-O-geranylgeranyl-sn-glycerol + diphosphate (EC 2.7.7.67) |
| No genes |
CDP-archaeol synthase:
2,3-bis-O-phytanyl-sn-glycerol 1-phosphate + CTP + H+→CDP-2,3-bis-O-phytanyl-sn-glycerol + diphosphate (EC 2.7.7.67) |
| No genes |
| CDP-2,3-bis-O-geranylgeranyl-sn-glycerol + L-serine→2,3-bis-O-geranylgeranyl-sn-glycero-1-phosphoserine + CMP + H+ (EC 2.7.8.38) |
| No genes |
| 2,3-bis-O-geranylgeranyl-sn-glycero-1-phosphoserine + H+→2,3-bis-O-geranylgeranyl-sn-glycero-1-phosphoethanolamine + CO2 |
| No genes |
| CDP-2,3-bis-O-phytanyl-sn-glycerol + L-serine→2,3-bis-O-phytanyl-sn-glycero-1-phosphoserine + CMP + H+ (EC 2.7.8.38) |
| No genes |
| 2,3-bis-O-phytanyl-sn-glycero-1-phosphoserine + H+→2,3-bis-O-phytanyl-sn-glycero-1-phosphoethanolamine + CO2 |
| No genes |
Links:
Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information