MetaCyc Pathway: toluene degradation VI (anaerobic) in Klebsiella michiganensis M5al

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Reactions and Genes 2-Deoxy-D-ribonic lithium salt 10 mM (C)
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Benzylsuccinate synthase (in reverse):
toluene + fumarate→(R)-2-benzylsuccinate (EC 4.1.99.11)
No genes
Succinyl-CoA:(R)-benzylsuccinate CoA-transferase:
(R)-2-benzylsuccinate + succinyl-CoA→(R)-benzylsuccinyl-CoA + succinate (EC 2.8.3.15)
No genes
(R)-benzylsuccinyl-CoA + an oxidized electron-transfer flavoprotein + H+→(E)-2-benzylidenesuccinyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.3)
No genes
(E)-2-benzylidenesuccinyl-CoA + H2O→(2S)-2-[(R)-α-hydroxybenzyl]succinyl-CoA (EC 4.2.1.180)
No genes
(2S)-2-[(R)-α-hydroxybenzyl]succinyl-CoA + NAD+→(S)-2-benzoylsuccinyl-CoA + NADH + H+ (EC 1.1.1.429)
No genes
(S)-2-benzoylsuccinyl-CoA + coenzyme A→benzoyl-CoA + succinyl-CoA (EC 2.3.1.310)
No genes
benzoyl-CoA + 2 ATP + 2 a reduced ferredoxin [iron-sulfur] cluster + 2 H2O→cyclohex-1,5-diene-1-carbonyl-CoA + 2 ADP + 2 an oxidized ferredoxin [iron-sulfur] cluster + 2 phosphate (EC 1.3.7.8)
No genes
Cyclohexa-1,5-dienecarbonyl-CoA hydratase (in reverse):
cyclohex-1,5-diene-1-carbonyl-CoA + H2O→6-hydroxycyclohex-1-ene-1-carbonyl-CoA (EC 4.2.1.100)
No genes
6-hydroxycyclohex-1-ene-1-carbonyl-CoA + NAD+→6-oxocyclohex-1-ene-1-carbonyl-CoA + NADH + H+ (EC 1.1.1.368)
No genes
6-oxocyclohex-1-ene-1-carbonyl-CoA + H2O→2-hydroxy-6-oxocyclohexane-1-carbonyl-CoA (EC 3.7.1.21)
No genes
2-hydroxy-6-oxocyclohexane-1-carbonyl-CoA + H2O→3-hydroxypimeloyl-CoA + H+ (EC 3.7.1.21)
No genes
3-hydroxypimeloyl-CoA dehydrogenase:
3-hydroxypimeloyl-CoA + NAD+→3-oxopimeloyl-CoA + NADH + H+ (EC 1.1.1.259)
No genes
3-oxopimeloyl-CoA + coenzyme A→glutaryl-CoA + acetyl-CoA
No genes
glutaryl-CoA + an oxidized electron-transfer flavoprotein + H+→(E)-glutaconyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.6)
No genes
(E)-glutaconyl-CoA + H+→crotonyl-CoA + CO2 (EC 1.3.8.6)
No genes
crotonyl-CoA + H2O→(S)-3-hydroxybutanoyl-CoA (EC 4.2.1.150)
BWI76_RS01365 -0.0
BWI76_RS01370 N.D.
BWI76_RS20455 +0.5
3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxybutanoyl-CoA + NAD+→acetoacetyl-CoA + NADH + H+ (EC 1.1.1.35)
BWI76_RS01365 -0.0
BWI76_RS01370 N.D.
BWI76_RS20455 +0.5
Acetyl-CoA C-acyltransferase (in reverse):
acetoacetyl-CoA + coenzyme A→2 acetyl-CoA (EC 2.3.1.16; 2.3.1.9)
BWI76_RS01360 +0.1
BWI76_RS13135 +0.6
BWI76_RS16135 +0.0
BWI76_RS20460 -0.2
BWI76_RS23445 -0.2
BWI76_RS23710 -3.8

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information