MetaCyc Pathway: propanoate fermentation to 2-methylbutanoate in Klebsiella michiganensis M5al

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Reactions and Genes 2-Deoxy-D-ribonic lithium salt 10 mM (C)
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Propionate CoA-transferase:
propanoate + acetyl-CoA→acetate + propanoyl-CoA (EC 2.8.3.1)
BWI76_RS23700 -4.5
Acetyl-CoA C-acyltransferase:
propanoyl-CoA + acetyl-CoA→2-methylacetoacetyl-CoA + coenzyme A (EC 2.3.1.16)
BWI76_RS01360 +0.1
BWI76_RS13135 +0.6
BWI76_RS16135 +0.0
BWI76_RS20460 -0.2
BWI76_RS23445 -0.2
BWI76_RS23710 -3.8
3-hydroxy-2-methylbutyryl-CoA dehydrogenase (in reverse):
2-methylacetoacetyl-CoA + NADH + H+→(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA + NAD+ (EC 1.1.1.178)
BWI76_RS01365 -0.0
BWI76_RS01370 N.D.
BWI76_RS20455 +0.5
(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA→(E)-2-methylcrotonoyl-CoA + H2O (EC 4.2.1.150)
BWI76_RS01365 -0.0
BWI76_RS01370 N.D.
BWI76_RS20455 +0.5
(E)-2-methylcrotonoyl-CoA + a reduced electron-transfer flavoprotein→(2S)-2-methylbutanoyl-CoA + an oxidized electron-transfer flavoprotein + H+ (EC 1.3.8.5)
No genes
(2S)-2-methylbutanoyl-CoA + H2O→2-methylbutanoate + coenzyme A + H+
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information