MetaCyc Pathway: 3-phenylpropanoate degradation in Klebsiella michiganensis M5al

Reactions and Genes	2-Deoxy-D-ribonic lithium salt 10 mM (C)
3-phenylpropanoate + ATP + coenzyme A→3-phenylpropanoyl-CoA + AMP + diphosphate
BWI76_RS17800	+0.1
3-phenylpropanoyl-CoA + an oxidized electron carrier→(E)-cinnamoyl-CoA + a reduced two electron carrier
No genes
Enoyl-CoA hydratase (in reverse): (E)-cinnamoyl-CoA + H2O→(S)-3-hydroxy-3-phenylpropanoyl-CoA (EC 4.2.1.17)
BWI76_RS01365	-0.0
BWI76_RS01370	N.D.
BWI76_RS13115	N.D.
BWI76_RS13120	N.D.
BWI76_RS20455	+0.5
3-hydroxyacyl-CoA dehydrogenase: (S)-3-hydroxy-3-phenylpropanoyl-CoA + NAD+→3-oxo-3-phenylpropanoyl-CoA + NADH + H+ (EC 1.1.1.35)
BWI76_RS01365	-0.0
BWI76_RS01370	N.D.
BWI76_RS20455	+0.5
Acetyl-CoA C-acyltransferase (in reverse): 3-oxo-3-phenylpropanoyl-CoA + coenzyme A→benzoyl-CoA + acetyl-CoA (EC 2.3.1.16)
BWI76_RS01360	+0.1
BWI76_RS13135	+0.6
BWI76_RS16135	+0.0
BWI76_RS20460	-0.2
BWI76_RS23445	-0.2
BWI76_RS23710	-3.8
Benzoyl-CoA 3-monooxygenase: benzoyl-CoA + NADPH + dioxygen + H+→3-hydroxybenzoyl-CoA + NADP+ + H2O (EC 1.14.13.58)
No genes
3-hydroxybenzoyl-CoA + a reduced two electron carrier + dioxygen→gentisate + an oxidized electron carrier + coenzyme A + H+
No genes
Gentisate 1,2-dioxygenase: gentisate + dioxygen→3-maleylpyruvate + H+ (EC 1.13.11.4)
BWI76_RS19585	+0.1
Maleylpyruvate isomerase: 3-maleylpyruvate→3-fumarylpyruvate (EC 5.2.1.4)
No genes
3-fumarylpyruvate + H2O→fumarate + pyruvate + H+ (EC 3.7.1.20)
BWI76_RS19580	+0.6

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information