MetaCyc Pathway: dTMP de novo biosynthesis (mitochondrial) in Pseudomonas fluorescens FW300-N2E2

Reactions and Genes	RCH2 defined with Cobalt chloride 0.04 mM
Dihydrofolate reductase (in reverse): a 7,8-dihydrofolate + NADPH + H+→a tetrahydrofolate + NADP+ (EC 1.5.1.3)
Pf6N2E2_4561	N.D.
Pf6N2E2_5274	+5.8
Glycine hydroxymethyltransferase: a tetrahydrofolate + L-serine→a 5,10-methylenetetrahydrofolate + glycine + H2O (EC 2.1.2.1)
Pf6N2E2_4717	-0.2
Pf6N2E2_5082	N.D.
Pf6N2E2_5083	-0.4
Pf6N2E2_5295	+0.3
Thymidylate synthase: a 5,10-methylenetetrahydrofolate + dUMP→a 7,8-dihydrofolate + dTMP (EC 2.1.1.45)
Pf6N2E2_3946	N.D.

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information