MetaCyc Pathway: methyl ketone biosynthesis (engineered) in Pseudomonas fluorescens SBW25-INTG

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Reactions and Genes p-Coumaric (C) and Ammonium chloride (N); with TAPS
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a fatty acid + ATP + coenzyme A→an acyl-CoA + AMP + diphosphate
No genes
Acyl-CoA oxidase:
a 2,3,4-saturated fatty acyl CoA + dioxygen→a (2E)-2-enoyl-CoA + hydrogen peroxide
(EC 1.3.3.6)
PFLU_RS15585 +0.0
Enoyl-CoA hydratase (in reverse):
a (2E)-2-enoyl-CoA + H2O→a 2,3,4-saturated (3S)-3-hydroxyacyl-CoA
(EC 4.2.1.17)
PFLU_RS07105 +0.2
PFLU_RS07650 -0.8
PFLU_RS08485 -0.7
PFLU_RS10835 -1.5
PFLU_RS12025 -3.6
PFLU_RS13550 -0.2
PFLU_RS14775 -3.6
PFLU_RS14785 +0.4
PFLU_RS16080 -4.8
PFLU_RS22820 -0.5
PFLU_RS22840 +0.2
PFLU_RS24260 +0.1
3-hydroxyacyl-CoA dehydrogenase:
a 2,3,4-saturated (3S)-3-hydroxyacyl-CoA + NAD+→a 2,3,4-saturated 3-oxoacyl-CoA + NADH + H+
(EC 1.1.1.35)
PFLU_RS07650 -0.8
PFLU_RS11090 -0.6
PFLU_RS22840 +0.2
a 2,3,4-saturated 3-oxoacyl-CoA + H2O→a 3-oxoacid + coenzyme A + H+
No genes
a 3-oxoacid + H+→a methyl ketone + CO2 (spontaneous)
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information