MetaCyc Pathway: 3-hydroxypropanoate/4-hydroxybutanate cycle in Pseudomonas fluorescens SBW25-INTG

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Reactions and Genes p-Coumaric (C) and Ammonium chloride (N); with TAPS
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Carbonate dehydratase (in reverse):
CO2 + H2O→hydrogen carbonate + H+ (EC 4.2.1.1)
PFLU_RS00275 +0.0
PFLU_RS03405 -0.0
PFLU_RS29995 -0.7
Biotin carboxylase:
hydrogen carbonate + ATP + a [biotin carboxyl-carrier-protein dimer]-N6-biotinyl-L-lysine→a [carboxyl-carrier protein dimer]-N6-carboxybiotinyl-L-lysine + ADP + H+ + phosphate (EC 6.3.4.14)
PFLU_RS03040 N.D.
PFLU_RS03045 N.D.
PFLU_RS09850 -0.0
PFLU_RS19645 -0.4
PFLU_RS19660 +0.4
PFLU_RS19665 -0.7
PFLU_RS26990 +0.3
a [carboxyl-carrier protein dimer]-N6-carboxybiotinyl-L-lysine + acetyl-CoA→malonyl-CoA + a [biotin carboxyl-carrier-protein dimer]-N6-biotinyl-L-lysine (EC 2.1.3.15)
PFLU_RS06355 N.D.
PFLU_RS20535 N.D.
malonyl-CoA + NADPH + H+→3-oxopropanoate + coenzyme A + NADP+ (EC 1.2.1.75)
No genes
3-oxopropanoate + NADPH + H+→3-hydroxypropanoate + NADP+ (EC 1.1.1.298)
No genes
3-hydroxypropanoate + ATP + coenzyme A→3-hydroxypropanoyl-CoA + AMP + diphosphate (EC 6.2.1.36)
No genes
3-hydroxypropanoyl-CoA→acryloyl-CoA + H2O (EC 4.2.1.116)
PFLU_RS14775 -0.5
acryloyl-CoA + NADPH + H+→propanoyl-CoA + NADP+ (EC 1.3.1.84)
No genes
Propionyl-CoA carboxylase:
hydrogen carbonate + propanoyl-CoA + ATP→(S)-methylmalonyl-CoA + ADP + H+ + phosphate (EC 6.4.1.3)
PFLU_RS06355 N.D.
PFLU_RS20535 N.D.
Methylmalonyl-CoA epimerase (in reverse):
(S)-methylmalonyl-CoA→(R)-methylmalonyl-CoA (EC 5.1.99.1)
No genes
Methylmalonyl-CoA mutase:
(R)-methylmalonyl-CoA→succinyl-CoA (EC 5.4.99.2)
No genes
succinyl-CoA + NADPH + H+→succinate semialdehyde + coenzyme A + NADP+ (EC 1.2.1.76)
No genes
succinate semialdehyde + NADPH + H+→4-hydroxybutanoate + NADP+ (EC 1.1.1.co)
No genes
4-hydroxybutanoate + ATP + coenzyme A→4-hydroxybutanoyl-CoA + AMP + diphosphate (EC 6.2.1.40)
No genes
4-hydroxybutanoyl-CoA→crotonyl-CoA + H2O (EC 4.2.1.120)
No genes
crotonyl-CoA + H2O→(S)-3-hydroxybutanoyl-CoA (EC 4.2.1.150)
PFLU_RS07105 -0.4
PFLU_RS07650 -1.0
PFLU_RS08485 -0.2
PFLU_RS10835 -0.8
PFLU_RS12025 -4.2
PFLU_RS24260 -0.3
3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxybutanoyl-CoA + NAD+→acetoacetyl-CoA + NADH + H+ (EC 1.1.1.35)
PFLU_RS07650 -1.0
PFLU_RS11090 -0.1
PFLU_RS22840 +0.1
Acetyl-CoA C-acyltransferase (in reverse):
acetoacetyl-CoA + coenzyme A→2 acetyl-CoA (EC 2.3.1.16; 2.3.1.9)
PFLU_RS03225 -0.9
PFLU_RS06735 -5.4
PFLU_RS07655 -1.1
PFLU_RS10575 +0.2
PFLU_RS14765 +0.1
PFLU_RS16065 +0.3
PFLU_RS17190 -0.4
PFLU_RS21180 -0.3
PFLU_RS22845 +0.2

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information