MetaCyc Pathway: colanic acid building blocks biosynthesis in Pseudomonas fluorescens SBW25-INTG

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Reactions and Genes p-Coumaric (C) and Ammonium chloride (N); with TAPS
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Glucose-6-phosphate isomerase:
α-D-glucose 6-phosphate→β-D-fructofuranose 6-phosphate (EC 5.3.1.9)
PFLU_RS25760 -5.1
Phosphoglucomutase (in reverse):
D-glucopyranose 6-phosphate→α-D-glucopyranose 1-phosphate (EC 5.4.2.2)
PFLU_RS15395 -2.6
PFLU_RS25845 N.D.
PFLU_RS29465 -0.8
UTP-monosaccharide-1-phosphate uridylyltransferase:
α-D-glucopyranose 1-phosphate + H+ + UTP→UDP-α-D-glucose + diphosphate (EC 2.7.7.64; 2.7.7.9)
PFLU_RS14550 -5.7
Mannose-6-phosphate isomerase (in reverse):
β-D-fructofuranose 6-phosphate→D-mannopyranose 6-phosphate (EC 5.3.1.8)
PFLU_RS02760 -1.4
PFLU_RS04860 +0.0
PFLU_RS09230 +0.2
PFLU_RS25010 +0.1
Phosphomannomutase (in reverse):
D-mannopyranose 6-phosphate→α-D-mannose 1-phosphate (EC 5.4.2.8)
PFLU_RS17945 N.D.
PFLU_RS29465 -0.8
UDP-glucose 4-epimerase:
UDP-α-D-glucose→UDP-α-D-galactose (EC 5.1.3.2)
PFLU_RS02390 N.D.
PFLU_RS11175 +0.7
PFLU_RS19285 +0.2
PFLU_RS23615 -0.3
UDP-glucose 6-dehydrogenase:
UDP-α-D-glucose + 2 NAD+ + H2O→UDP-α-D-glucuronate + 2 NADH + 3 H+ (EC 1.1.1.22)
PFLU_RS14800 -0.8
PFLU_RS17930 -0.1
Mannose-1-phosphate guanylyltransferase:
α-D-mannose 1-phosphate + GTP + H+→GDP-α-D-mannose + diphosphate (EC 2.7.7.13)
PFLU_RS04860 +0.0
PFLU_RS17870 -0.0
PFLU_RS27440 -0.5
GDP-mannose 4,6-dehydratase:
GDP-α-D-mannose→GDP-4-dehydro-α-D-rhamnose + H2O (EC 4.2.1.47)
PFLU_RS17890 +0.4
GDP-L-fucose synthase:
GDP-4-dehydro-α-D-rhamnose→GDP-4-dehydro-6-deoxy-β-L-galactose (EC 1.1.1.271)
PFLU_RS17885 -0.5
GDP-L-fucose synthase (in reverse):
GDP-4-dehydro-6-deoxy-β-L-galactose + NADPH + H+→GDP-β-L-fucose + NADP+ (EC 1.1.1.271)
PFLU_RS17885 -0.5

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information