MetaCyc Pathway: L-isoleucine biosynthesis V in Pseudomonas stutzeri RCH2

Reactions and Genes	Bacitracin 0.25 mg/ml
Acetate--CoA ligase: 2-methylbutanoate + ATP + coenzyme A→(2S)-2-methylbutanoyl-CoA + AMP + diphosphate (EC 6.2.1.1)
Psest_2220	+0.5
Psest_2657	-0.1
3-methyl-2-oxobutanoate dehydrogenase (ferredoxin) (in reverse): (2S)-2-methylbutanoyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→(3S)-3-methyl-2-oxopentanoate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster (EC 1.2.7.7)
Psest_3517	-0.2
Branched-chain-amino-acid transaminase (in reverse): (3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
Psest_0450	+1.7

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information