MetaCyc Pathway: L-isoleucine degradation I in Pseudomonas stutzeri RCH2

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Reactions and Genes Bacitracin 0.25 mg/ml
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Branched-chain-amino-acid transaminase:
L-isoleucine + 2-oxoglutarate→(3S)-3-methyl-2-oxopentanoate + L-glutamate (EC 2.6.1.42)
Psest_0450 +1.7
2-oxoisovalerate dehydrogenase (acylating):
(3S)-3-methyl-2-oxopentanoate + coenzyme A + NAD+→(2S)-2-methylbutanoyl-CoA + CO2 + NADH (EC 1.2.1.25)
No genes
(2S)-2-methylbutanoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(E)-2-methylcrotonoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.5)
Psest_2440 -0.0
Psest_2445 -0.3
(E)-2-methylcrotonoyl-CoA + H2O→(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA (EC 4.2.1.150)
Psest_2654 N.D.
Psest_3109 +0.2
Psest_3969 -0.4
Psest_4235 -0.0
3-hydroxy-2-methylbutyryl-CoA dehydrogenase:
(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA + NAD+→2-methylacetoacetyl-CoA + NADH + H+ (EC 1.1.1.178)
Psest_2447 -0.4
Psest_2654 N.D.
Psest_3109 +0.2
Psest_3800 -0.0
Psest_3969 -0.4
Psest_4235 -0.0
Acetyl-CoA C-acyltransferase (in reverse):
2-methylacetoacetyl-CoA + coenzyme A→acetyl-CoA + propanoyl-CoA (EC 2.3.1.16)
Psest_1076 +0.2
Psest_1829 -0.2
Psest_2446 +0.4
Psest_2476 -0.3
Psest_2653 N.D.
Psest_3070 -1.2

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information