MetaCyc Pathway: superpathway of L-isoleucine biosynthesis I in Enterobacter sp. TBS_079

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Reactions and Genes Putrescine (N)
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Aspartate transaminase (in reverse):
oxaloacetate + L-glutamate→L-aspartate + 2-oxoglutarate (EC 2.6.1.1)
MPMX20_00609 +0.0
MPMX20_00612 +0.1
MPMX20_01049 -0.1
MPMX20_01059 +0.1
MPMX20_01157 -0.0
MPMX20_01586 -0.9
MPMX20_02048 -0.1
MPMX20_02230 -0.1
MPMX20_02425 -0.2
MPMX20_03220 -1.0
MPMX20_04048 +0.0
Aspartate kinase:
L-aspartate + ATP→L-aspartyl-4-phosphate + ADP (EC 2.7.2.4)
MPMX20_00240 -0.1
MPMX20_00710 -4.4
MPMX20_04530 +0.1
Aspartate-semialdehyde dehydrogenase (in reverse):
L-aspartyl-4-phosphate + NADPH + H+→L-aspartate 4-semialdehyde + NADP+ + phosphate (EC 1.2.1.11)
MPMX20_02127 -0.0
MPMX20_03254 +0.6
MPMX20_04315 N.D.
Homoserine dehydrogenase (in reverse):
L-aspartate 4-semialdehyde + NAD(P)H + H+→L-homoserine + NAD(P)+ (EC 1.1.1.3)
MPMX20_00710 -4.4
MPMX20_04530 +0.1
Homoserine kinase:
L-homoserine + ATP→O-phospho-L-homoserine + ADP + H+ (EC 2.7.1.39)
MPMX20_00711 -5.3
Threonine synthase:
O-phospho-L-homoserine + H2O→L-threonine + phosphate (EC 4.2.3.1)
MPMX20_00712 -5.2
Threonine ammonia-lyase:
L-threonine→(Z)-2-aminobutenoate + H2O (EC 4.3.1.19)
MPMX20_04021 +0.0
MPMX20_04498 -2.7
1-aminocyclopropane-1-carboxylate deaminase:
(Z)-2-aminobutenoate→2-iminobutanoate (spontaneous) (EC 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
MPMX20_01953 +0.0
MPMX20_04021 +0.0
MPMX20_04498 -2.7
2-iminobutanoate + H2O→2-oxobutanoate + ammonium (spontaneous) (EC 3.5.99.10; 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
MPMX20_00542 -0.3
MPMX20_01953 +0.0
MPMX20_04021 +0.0
MPMX20_04498 -2.7
Acetolactate synthase:
2-oxobutanoate + H+ + pyruvate→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)
MPMX20_00031 -0.9
MPMX20_00032 -1.1
MPMX20_00771 +0.6
MPMX20_00772 +0.5
MPMX20_01224 -0.2
MPMX20_03783 +0.0
MPMX20_04501 -0.1
MPMX20_04502 -0.3
Ketol-acid reductoisomerase (in reverse):
(S)-2-aceto-2-hydroxybutanoate + NADPH + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NADP+ (EC 1.1.1.86)
MPMX20_04496 -5.0
Dihydroxy-acid dehydratase:
(R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)
MPMX20_00552 -0.2
MPMX20_04499 -5.4
Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
MPMX20_04500 -0.6

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information