MetaCyc Pathway: superpathway of L-threonine metabolism in Phocaeicola vulgatus CL09T03C04

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Reactions and Genes Ammonium chloride (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
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Threonine ammonia-lyase:
L-threonine→(Z)-2-aminobutenoate + H2O (EC 4.3.1.19)
HMPREF1058_RS06355 +1.7
L-threonine + ATP→L-threonine 3-O-phosphate + ADP + H+ (EC 2.7.1.177)
No genes
L-threonine 3-dehydrogenase:
L-threonine + NAD+→L-2-amino-3-oxobutanoate + NADH + H+ (EC 1.1.1.103)
HMPREF1058_RS10460 -0.4
L-threonine→acetaldehyde + glycine (EC 4.1.2.48; 4.1.2.5)
HMPREF1058_RS12955 +0.2
Threonine-phosphate decarboxylase:
L-threonine 3-O-phosphate + H+→(R)-1-amino-2-propanol O-2-phosphate + CO2 (EC 4.1.1.81)
HMPREF1058_RS01390 -1.9
Acetaldehyde dehydrogenase (acetylating):
acetaldehyde + coenzyme A + NAD+→acetyl-CoA + NADH + H+ (EC 1.2.1.10)
No genes
Glycine C-acetyltransferase (in reverse):
L-2-amino-3-oxobutanoate + coenzyme A→glycine + acetyl-CoA (EC 2.3.1.29)
HMPREF1058_RS10455 -0.3
1-aminocyclopropane-1-carboxylate deaminase:
(Z)-2-aminobutenoate→2-iminobutanoate (spontaneous) (EC 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
HMPREF1058_RS06355 +1.7
L-threonine 3-dehydrogenase:
L-2-amino-3-oxobutanoate + H+→aminoacetone + CO2 (spontaneous) (EC 1.1.1.103; 1.1.1.381)
HMPREF1058_RS10460 -0.4
Primary-amine oxidase:
aminoacetone + dioxygen + H2O→methylglyoxal + ammonium + hydrogen peroxide (EC 1.4.3.21)
No genes
2-iminobutanoate + H2O→2-oxobutanoate + ammonium (spontaneous) (EC 3.5.99.10; 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
HMPREF1058_RS06355 +1.7
HMPREF1058_RS18400 +0.7
Acetolactate synthase:
2-oxobutanoate + H+ + pyruvate→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)
HMPREF1058_RS16820 -2.3
HMPREF1058_RS16825 -2.0
Formate C-acetyltransferase (in reverse):
2-oxobutanoate + coenzyme A→propanoyl-CoA + formate (EC 2.3.1.54)
HMPREF1058_RS12515 -0.2
HMPREF1058_RS18120 +0.3
Ketol-acid reductoisomerase (in reverse):
(S)-2-aceto-2-hydroxybutanoate + NADPH + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NADP+ (EC 1.1.1.86)
HMPREF1058_RS16835 -2.2
propanoyl-CoA + phosphate→propanoyl phosphate + coenzyme A (EC 2.3.1.222)
HMPREF1058_RS08505 N.D.
Dihydroxy-acid dehydratase:
(R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)
HMPREF1058_RS16815 -2.6
Acetate kinase (in reverse):
propanoyl phosphate + ADP→propanoate + ATP (EC 2.7.2.1; 2.7.2.14; 2.7.2.15; 2.7.2.7)
HMPREF1058_RS08510 N.D.
HMPREF1058_RS12040 -0.0
Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
HMPREF1058_RS02220 -2.4

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information