MetaCyc Pathway: hexitol fermentation to lactate, formate, ethanol and acetate in Shewanella oneidensis MR-1

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Reactions and Genes Fumarate, anaerobic, 1G configuration
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Mannitol-1-phosphate 5-dehydrogenase:
D-mannitol 1-phosphate + NAD+→β-D-fructofuranose 6-phosphate + NADH + H+ (EC 1.1.1.17)
No genes
Sorbitol-6-phosphate 2-dehydrogenase:
D-sorbitol 6-phosphate + NAD+→keto-D-fructose 6-phosphate + NADH + H+ (EC 1.1.1.140)
No genes
Fructose-bisphosphatase:
β-D-fructofuranose 1,6-bisphosphate + H2O→β-D-fructofuranose 6-phosphate + phosphate (EC 3.1.3.11)
SO3991 (fbp) N.D.
6-phosphofructokinase:
β-D-fructofuranose 6-phosphate + ATP→β-D-fructofuranose 1,6-bisphosphate + ADP + H+ (EC 2.7.1.11)
No genes
Fructose-bisphosphate aldolase:
β-D-fructofuranose 1,6-bisphosphate→glycerone phosphate + D-glyceraldehyde 3-phosphate (EC 4.1.2.13)
SO0933 (fba) N.D.
Triose-phosphate isomerase (in reverse):
glycerone phosphate→D-glyceraldehyde 3-phosphate (EC 5.3.1.1)
SO1200 (tpiA) N.D.
Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating):
D-glyceraldehyde 3-phosphate + NAD+ + phosphate→3-phospho-D-glyceroyl phosphate + NADH + H+ (EC 1.2.1.12)
SO0538 (gapA-1) +0.1
SO2345 (gapA-2) N.D.
SO2347 (gapA-3) -2.3
Phosphoglycerate kinase (in reverse):
3-phospho-D-glyceroyl phosphate + ADP→3-phospho-D-glycerate + ATP (EC 2.7.2.3)
SO0932 (pgk) N.D.
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.12)
SO0049 (gpmA) N.D.
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.11)
No genes
Phosphopyruvate hydratase:
2-phospho-D-glycerate→phosphoenolpyruvate + H2O (EC 4.2.1.11)
SO3440 (eno) N.D.
Pyruvate, water dikinase:
pyruvate + ATP + H2O→phosphoenolpyruvate + AMP + 2 H+ + phosphate (EC 2.7.9.2)
SO2644 (ppsA) -5.3
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
SO2491 (pykA) -1.9
L-lactate dehydrogenase (in reverse):
pyruvate + NADH + H+→(S)-lactate + NAD+ (EC 1.1.1.27)
No genes
Formate C-acetyltransferase (in reverse):
pyruvate + coenzyme A→acetyl-CoA + formate (EC 2.3.1.54)
SO2912 (pflB) -1.4
SO2913 (pflA) -1.3
Acetaldehyde dehydrogenase (acetylating) (in reverse):
acetyl-CoA + NADH + H+→acetaldehyde + coenzyme A + NAD+ (EC 1.2.1.10)
SO2136 (adhE) -0.1
Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
SO2916 (pta) -1.7
Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
SO2915 (ackA) -1.7
SO3613 (purT) +0.0
Alcohol dehydrogenase (in reverse):
acetaldehyde + NADH + H+→ethanol + NAD+ (EC 1.1.1.1)
SO1490 (adhB) +0.1
SO2054 (adhC) -0.1
SO2136 (adhE) -0.1
SO2477 -0.4
SOA0161 -1.1
SOA0164 +0.0

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information