MetaCyc Pathway: L-lysine biosynthesis III in Klebsiella michiganensis M5al

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Reactions and Genes RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
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Aspartate kinase:
L-aspartate + ATP→L-aspartyl-4-phosphate + ADP
(EC 2.7.2.4)
BWI76_RS00790 +0.3
BWI76_RS01790 -1.6
BWI76_RS04245 -2.6
Aspartate-semialdehyde dehydrogenase (in reverse):
L-aspartyl-4-phosphate + NADPH + H+→L-aspartate 4-semialdehyde + NADP+ + phosphate
(EC 1.2.1.11)
BWI76_RS12880 -0.2
BWI76_RS20385 +1.0
BWI76_RS26220 N.D.
L-aspartate 4-semialdehyde + pyruvate→(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H+ + H2O (EC 4.3.3.7)
BWI76_RS00585 -0.1
BWI76_RS05685 +0.3
BWI76_RS10255 +0.3
BWI76_RS15245 +0.0
BWI76_RS18305 +0.5
BWI76_RS20905 N.D.
BWI76_RS26315 -0.1
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H + H+→(S)-2,3,4,5-tetrahydrodipicolinate + NAD(P)+ + H2O (EC 1.17.1.8)
BWI76_RS04460 N.D.
(S)-2,3,4,5-tetrahydrodipicolinate + H+ + H2O→L-α-amino-ε-keto-pimelate (spontaneous)
No genes
Diaminopimelate dehydrogenase (in reverse):
L-α-amino-ε-keto-pimelate + ammonium + NADPH + H+meso-diaminopimelate + NADP+ + H2O
(EC 1.4.1.16)
No genes
Diaminopimelate decarboxylase:
meso-diaminopimelate + H+→L-lysine + CO2
(EC 4.1.1.20)
BWI76_RS23360 -3.8

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information