MetaCyc Pathway: superpathway of aromatic amino acid biosynthesis in Klebsiella michiganensis M5al

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Reactions and Genes RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
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3-deoxy-7-phosphoheptulonate synthase:
D-erythrose 4-phosphate + phosphoenolpyruvate + H2O→3-deoxy-D-arabino-heptulosonate 7-phosphate + phosphate (EC 2.5.1.54)
BWI76_RS08600 -0.3
BWI76_RS15085 +0.1
BWI76_RS17075 +0.4
BWI76_RS21480 -1.6
3-dehydroquinate synthase:
3-deoxy-D-arabino-heptulosonate 7-phosphate→3-dehydroquinate + phosphate (EC 4.2.3.4)
BWI76_RS26085 -4.2
3-dehydroquinate dehydratase:
3-dehydroquinate→3-dehydroshikimate + H2O (EC 4.2.1.10)
BWI76_RS04645 -4.9
BWI76_RS22790 N.D.
Shikimate dehydrogenase (in reverse):
3-dehydroshikimate + NADPH + H+→shikimate + NADP+ (EC 1.1.1.25)
BWI76_RS01715 +0.4
BWI76_RS04655 +0.4
BWI76_RS06460 +0.1
BWI76_RS16935 +0.4
BWI76_RS25645 -2.3
Shikimate kinase:
shikimate + ATP→shikimate 3-phosphate + ADP + H+ (EC 2.7.1.71)
BWI76_RS06185 -1.5
BWI76_RS26090 N.D.
3-phosphoshikimate 1-carboxyvinyltransferase:
shikimate 3-phosphate + phosphoenolpyruvate→5-enolpyruvoyl-shikimate 3-phosphate + phosphate (EC 2.5.1.19)
BWI76_RS09760 -4.4
Chorismate synthase:
5-enolpyruvoyl-shikimate 3-phosphate→chorismate + phosphate (EC 4.2.3.5)
BWI76_RS20435 -3.1
Anthranilate synthase:
chorismate + L-glutamine→anthranilate + L-glutamate + H+ + pyruvate (EC 4.1.3.27)
BWI76_RS11855 -1.2
BWI76_RS11860 -1.8
BWI76_RS14505 +0.1
Chorismate mutase:
chorismate→prephenate (EC 5.4.99.5)
BWI76_RS21465 -1.5
BWI76_RS21475 -2.9
Prephenate dehydratase:
prephenate + H+→3-phenyl-2-oxopropanoate + CO2 + H2O (EC 4.2.1.51)
BWI76_RS21465 -1.5
BWI76_RS21950 +0.1
Prephenate dehydrogenase:
prephenate + NAD+→3-(4-hydroxyphenyl)pyruvate + CO2 + NADH (EC 1.3.1.12)
BWI76_RS21475 -2.9
Anthranilate phosphoribosyltransferase (in reverse):
anthranilate + 5-phospho-α-D-ribose 1-diphosphate→N-(5-phosphoribosyl)-anthranilate + diphosphate (EC 2.4.2.18)
BWI76_RS08925 +0.1
BWI76_RS11855 -1.2
Phosphoribosylanthranilate isomerase:
N-(5-phosphoribosyl)-anthranilate→1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate (EC 5.3.1.24)
BWI76_RS11850 -1.6
Aspartate transaminase (in reverse):
3-phenyl-2-oxopropanoate + L-glutamate→L-phenylalanine + 2-oxoglutarate (EC 2.6.1.1; 2.6.1.27; 2.6.1.57)
BWI76_RS00970 -1.0
BWI76_RS01925 -1.5
BWI76_RS03690 +0.1
BWI76_RS06660 -0.0
BWI76_RS06750 -0.0
BWI76_RS07560 +0.3
BWI76_RS07570 +0.3
BWI76_RS08675 -0.2
BWI76_RS09870 -1.8
BWI76_RS10245 -0.8
BWI76_RS12985 -0.6
BWI76_RS13400 -0.4
BWI76_RS18930 -3.4
BWI76_RS20205 -0.7
BWI76_RS22035 +0.3
BWI76_RS24235 +0.3
Aspartate transaminase (in reverse):
3-(4-hydroxyphenyl)pyruvate + L-glutamate→L-tyrosine + 2-oxoglutarate (EC 2.6.1.1; 2.6.1.27; 2.6.1.5; 2.6.1.57)
BWI76_RS01925 -1.5
BWI76_RS03690 +0.1
BWI76_RS06660 -0.0
BWI76_RS06750 -0.0
BWI76_RS07560 +0.3
BWI76_RS07570 +0.3
BWI76_RS08675 -0.2
BWI76_RS09870 -1.8
BWI76_RS10245 -0.8
BWI76_RS12985 -0.6
BWI76_RS13400 -0.4
BWI76_RS18930 -3.4
BWI76_RS20205 -0.7
BWI76_RS22035 +0.3
BWI76_RS24235 +0.3
Indole-3-glycerol-phosphate synthase:
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate + H+→(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O (EC 4.1.1.48)
BWI76_RS11850 -1.6
Indole-3-glycerol-phosphate lyase:
(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate→indole + D-glyceraldehyde 3-phosphate (EC 4.1.2.8; 4.2.1.20)
BWI76_RS11840 -1.4
BWI76_RS11845 -1.7
indole + L-serine→L-tryptophan + H2O (EC 4.2.1.122; 4.2.1.20)
BWI76_RS11840 -1.4
BWI76_RS11845 -1.7

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information