MetaCyc Pathway: L-lysine biosynthesis I in Klebsiella michiganensis M5al

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Reactions and Genes RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x
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Aspartate kinase:
L-aspartate + ATP→L-aspartyl-4-phosphate + ADP
(EC 2.7.2.4)
BWI76_RS00790 -0.1
BWI76_RS01790 -1.2
BWI76_RS04245 -1.4
Aspartate-semialdehyde dehydrogenase (in reverse):
L-aspartyl-4-phosphate + NADPH + H+→L-aspartate 4-semialdehyde + NADP+ + phosphate
(EC 1.2.1.11)
BWI76_RS12880 +0.2
BWI76_RS20385 +0.8
BWI76_RS26220 N.D.
L-aspartate 4-semialdehyde + pyruvate→(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H+ + H2O (EC 4.3.3.7)
BWI76_RS00585 +0.0
BWI76_RS05685 -0.4
BWI76_RS10255 +1.1
BWI76_RS15245 -0.2
BWI76_RS18305 +1.6
BWI76_RS20905 N.D.
BWI76_RS26315 +0.1
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H + H+→(S)-2,3,4,5-tetrahydrodipicolinate + NAD(P)+ + H2O (EC 1.17.1.8)
BWI76_RS04460 N.D.
2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase:
(S)-2,3,4,5-tetrahydrodipicolinate + succinyl-CoA + H2ON-succinyl-2-amino-6-ketopimelate + coenzyme A
(EC 2.3.1.117)
BWI76_RS05195 N.D.
Succinyldiaminopimelate transaminase (in reverse):
N-succinyl-2-amino-6-ketopimelate + L-glutamateN-succinyl-L,L-2,6-diaminopimelate + 2-oxoglutarate
(EC 2.6.1.17)
BWI76_RS09755 -4.0
BWI76_RS26000 -0.8
Succinyl-diaminopimelate desuccinylase:
N-succinyl-L,L-2,6-diaminopimelate + H2O→L,L-diaminopimelate + succinate
(EC 3.5.1.18)
BWI76_RS10270 -0.1
BWI76_RS13530 +0.5
BWI76_RS14620 -0.4
BWI76_RS20870 N.D.
Diaminopimelate epimerase:
L,L-diaminopimelate→meso-diaminopimelate
(EC 5.1.1.7)
BWI76_RS01200 +0.8
BWI76_RS18105 N.D.
Diaminopimelate decarboxylase:
meso-diaminopimelate + H+→L-lysine + CO2
(EC 4.1.1.20)
BWI76_RS23360 -4.2

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information