MetaCyc Pathway: pyruvate fermentation to butanol II (engineered) in Pseudomonas fluorescens SBW25-INTG

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Reactions and Genes malate (C) and Ammonium chloride (N); with TAPS
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Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
PFLU_RS03225 -0.1
PFLU_RS06735 +0.2
PFLU_RS07655 -0.1
PFLU_RS10575 +0.0
PFLU_RS14765 +0.1
PFLU_RS16065 -0.1
PFLU_RS17190 -0.8
PFLU_RS21180 -0.2
PFLU_RS22845 +0.0
3-hydroxyacyl-CoA dehydrogenase (in reverse):
acetoacetyl-CoA + NADH + H+→(S)-3-hydroxybutanoyl-CoA + NAD+ (EC 1.1.1.35)
PFLU_RS07650 -0.2
PFLU_RS11090 +0.0
PFLU_RS22840 +0.0
(S)-3-hydroxybutanoyl-CoA→crotonyl-CoA + H2O (EC 4.2.1.150)
PFLU_RS07105 +0.1
PFLU_RS07650 -0.2
PFLU_RS08485 -0.7
PFLU_RS10835 -0.5
PFLU_RS12025 -1.6
PFLU_RS24260 -0.0
Trans-2-enoyl-CoA reductase (NAD(+)) (in reverse):
crotonyl-CoA + NADH + H+→butanoyl-CoA + NAD+ (EC 1.3.1.44)
PFLU_RS04175 -0.3
PFLU_RS09195 -0.2
PFLU_RS14770 -0.3
PFLU_RS14780 -0.0
PFLU_RS17205 +0.0
PFLU_RS18005 -0.4
PFLU_RS22410 +0.2
Butanal dehydrogenase (in reverse):
butanoyl-CoA + NAD(P)H + H+→1-butanal + coenzyme A + NAD(P)+ (EC 1.2.1.57)
No genes
1-butanal + NADH + H+→butan-1-ol + NAD+
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information