Fitness data for SGL_RS06660 in Synechocystis sp000284455 PCC 6803

DNA helicase PcrA
SEED: ATP-dependent DNA helicase UvrD/PcrA
KEGG: DNA helicase II / ATP-dependent DNA helicase PcrA

Top 20 experiments with the strongest phenotypes (|fitness| ≥ 0.2)

group	condition	fitness	t score
carbon dioxide	growth in full media bubbled with ambient air	-1.8	-6.1	compare
carbon dioxide	growth in full media bubbled with ambient air	-0.8	-2.9	compare
nitrogen source	Arg 10 mM as N source, buffered with TES 20 mM TES pH8 but diluted to prevent light attenuation	-0.3	-1.7	compare
carbon dioxide	growth in media lacking HCO3 buffered with 10 TES pH8 and bubbled with air enriched with 5% CO2 but diluted to prevent light attenuation	-0.3	-1.7	compare
carbon dioxide	growth in full media bubbled with ambient air but diluted to prevent light attenuation	-0.3	-1.3	compare
photoheterotrophic	growth in full media and 10 mM glucose= photoheterotrophic growth	-0.3	-1.7	compare
carbon dioxide	growth in full media bubbled with air enriched in 5% CO2 but diluted to prevent light attenuation	-0.2	-1.1	compare
carbon dioxide	growth in full media bubbled with air enriched in 1% CO2 but diluted to prevent light attenuation	-0.2	-1.2	compare
carbon dioxide	growth in full media bubbled with air enriched in 5% CO2	-0.2	-1.4	compare
metal stress	Synechocystis_PCC6803_ML3a diversity barseq (outgrowth of original library)	-0.2	-1.1	compare
metal stress	growth in full media supplemented with 5 .M CuSO4	+0.2	+1.7	compare
metal stress	growth in full media supplemented with 3 .M CuSO4	+0.2	+1.4	compare
metal stress	growth in full media supplemented with 300 .M BCSA to remove any trace of Cu but diluted to prevent light attenuation	+0.2	+1.5	compare
nitrogen source	NaNO2 10 mM as N source, buffered with 20 mM TES pH8	+0.3	+1.8	compare
photoheterotrophic	growth in full media and 10 mM glucose= photoheterotrophic growth but diluted to prevent slight attenuation and replinshing of glucose	+0.3	+1.6	compare
nitrogen source	Inoculated in media lacking a N source and incubated for 1 week, NaNo3 added and recovered for 3 days	+0.3	+2.0	compare
metal stress	growth in full media supplemented with 5 .M CuSO4	+0.3	+2.7	compare
metal stress	growth in full media supplemented with 3 .M CuSO4 but diluted to prevent light attenuation	+0.4	+2.3	compare
glucose	Na NO2 10 mM as N source, buffered with TES 20 mM TES pH8 but diluted to prevent light attenuation	+0.5	+3.5	compare
nitrogen source	growth in full media and 10 mM glucose= photomixotrophic growth	+2.6	+10.9	compare