Fitness data for BPHYT_RS25505 in Burkholderia phytofirmans PsJN

RNA polymerase sigma factor
SEED: DNA-directed RNA polymerase specialized sigma subunit, sigma24-like
KEGG: RNA polymerase sigma-70 factor, ECF subfamily

Top 20 experiments with the strongest phenotypes (|fitness| ≥ 0.6)


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group condition fitness t score  
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.8 -1.1 compare
phage 24hr PHYTO7 MOI 1 -0.7 -1.2 compare
phage 24hr PHYTO7 MOI 10 -0.6 -1.8 compare
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -0.6 -1.5 compare
phage 24hr PHYTO2 MOI 0.1 +0.6 +1.6 compare
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.6 +1.7 compare
phage 24hr PHYTO4 MOI 10 +0.6 +1.4 compare
carbon source 2-Deoxy-D-Ribose (C) +0.6 +2.3 compare
carbon source 2-Deoxy-D-Ribose (C) +0.7 +1.8 compare
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.7 +2.0 compare
phage 24hr PHYTO3 MOI 10 +0.7 +2.0 compare
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.7 +2.2 compare
motility outer cut, LB soft agar motility assay +0.7 +1.9 compare
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB +0.7 +2.1 compare
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.7 +2.6 compare
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.7 +1.3 compare
phage 24hr PHYTO6 MOI 0.1 +0.8 +2.9 compare
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.8 +2.2 compare
motility inner cut, LB soft agar motility assay +1.0 +3.9 compare
supernatant control Vogels_fungal_media; pH 5.5 +1.1 +2.7 compare