Fitness data for BPHYT_RS19575 in Burkholderia phytofirmans PsJN

tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC
SEED: tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (EC 2.1.1.61)

Top 20 experiments with the strongest phenotypes (|fitness| ≥ 0.4)


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group condition fitness t score  
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.9 -3.4 compare
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days -0.6 -2.9 compare
phage 24hr PHYTO11 MOI 1 -0.6 -2.2 compare
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.6 -2.5 compare
phage 24hr PHYTO11 MOI 0.1 -0.5 -2.5 compare
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.5 -1.8 compare
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 -0.5 -1.9 compare
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB -0.5 -1.5 compare
phage 36hr PHYTO4 MOI 0.1 -0.4 -2.5 compare
motility inner cut, LB soft agar motility assay -0.4 -1.7 compare
motility outer cut, LB soft agar motility assay -0.4 -2.1 compare
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -0.4 -1.7 compare
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -0.4 -1.8 compare
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.4 -1.9 compare
phage 24hr PHYTO6 MOI 0.1 -0.4 -2.2 compare
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 +0.4 +1.0 compare
vitamin No vitamins t1 +0.4 +2.3 compare
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days +0.4 +1.0 compare
carbon source D-Glucose (C) +0.5 +1.8 compare
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.5 +1.8 compare