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Fitness for 5 genes in
Synechocystis sp000284455 PCC 6803
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
SGL_RS18470 and SGL_RS19675 are separated by 102 nucleotides
SGL_RS19675 and SGL_RS00135 are separated by 213 nucleotides
SGL_RS00135 and SGL_RS00140 overlap by 48 nucleotides
SGL_RS00140 and SGL_RS00145 are separated by 28 nucleotides
SGL_RS18470: SGL_RS18470 - HNH endonuclease, at 30,281 to 30,574
_RS18470
SGL_RS19675: SGL_RS19675 - hypothetical protein, at 30,677 to 31,183
_RS19675
SGL_RS00135: SGL_RS00135 - IS701 family transposase, at 31,397 to 32,062
_RS00135
SGL_RS00140: SGL_RS00140 - ARMT1-like domain-containing protein, at 32,015 to 32,836
_RS00140
SGL_RS00145: SGL_RS00145 - DUF4079 domain-containing protein, at 32,865 to 33,350
_RS00145
Group
Condition
SGL
_RS18470
SGL
_RS19675
SGL
_RS00135
SGL
_RS00140
SGL
_RS00145
metal stress
growth in full media supplemented with 2mM sodum metaarsenite but diluted to prevent light attenuation
-0.1
-0.1
N.D.
+0.1
-0.5
metal stress
growth in full media supplemented with 5mM sodum metaarsenite but diluted to prevent light attenuation
-0.0
-0.1
N.D.
-0.1
-0.6
nitrogen source
NaNO2 10 mM as N source, buffered with 20 mM TES pH8
+0.1
-0.1
N.D.
-0.3
-0.4
metal stress
growth in full media supplemented with 3 .M Cl2Co but diluted to prevent light attenuation
-0.1
-0.0
N.D.
-0.1
-0.5
metal stress
growth in full media supplemented with 5 .M Cl2Co
+0.1
-0.1
N.D.
-0.2
-0.3
metal stress
growth in full media supplemented with 5mM sodum metaarsenite
-0.3
-0.1
N.D.
-0.1
+0.3
metal stress
growth in full media supplemented with 5 .M Cl2Co
-0.5
+0.1
N.D.
-0.0
+0.2
nitrogen source
growth in full media and 10 mM glucose= photoheterotrophic growth
-0.2
-0.1
N.D.
-0.2
+0.2
metal stress
growth in full media supplemented with 3 .M CuSO4
+0.0
+0.2
N.D.
+0.2
-0.4
metal stress
growth in full media supplemented with 5mM sodum metaarsenite but diluted to prevent light attenuation
-0.4
+0.1
N.D.
+0.1
+0.3
nitrogen source
Inoculated in media lacking a N source and incubated for 1 week, NaNo3 added and recovered for 3 days
-0.2
-0.0
N.D.
-0.3
+0.6
nitrogen source
NaNO3 10 mM as N source, buffered with TES 20 mM TES pH8
+0.2
-0.3
N.D.
-0.2
+0.4
nitrogen source
NaNO3 10 mM as N source, buffered with 20 mM TES pH8
-0.0
-0.0
N.D.
-0.3
+0.4
metal stress
Synechocystis_PCC6803_ML3a diversity barseq (outgrowth of original library)
+0.4
-0.0
N.D.
-0.1
-0.1
metal stress
growth in full media supplemented with 5 .M Cl2Co but diluted to prevent light attenuation
-0.2
-0.2
N.D.
+0.1
+0.4
nitrogen source
Arg 10 mM as N source, buffered with TES 20 mM TES pH8 but diluted to prevent light attenuation
-0.2
+0.0
N.D.
-0.1
+0.5
glucose
Na NO2 10 mM as N source, buffered with TES 20 mM TES pH8 but diluted to prevent light attenuation
+0.1
-0.0
N.D.
-0.2
+0.4
carbon dioxide
growth in media lacking HCO3 buffered with 10 TES pH8 and bubbled with air enriched with 5% CO2 but diluted to prevent light attenuation
-0.0
+0.2
N.D.
+0.3
-0.2
carbon dioxide
growth in full media bubbled with ambient air but diluted to prevent light attenuation
+0.2
+0.3
N.D.
-0.3
+0.2
carbon dioxide
growth in media lacking HCO3 buffered with 10 TES pH8 and bubbled with ambient air
+0.6
+0.1
N.D.
-0.0
-0.2
photoheterotrophic
growth in full media and 10 mM glucose= photoheterotrophic growth
+0.4
-0.2
N.D.
+0.0
+0.3
metal stress
growth in full media supplemented with 5 .M CuSO4
-0.0
-0.0
N.D.
+0.3
+0.4
metal stress
growth in full media supplemented with 3 .M CuSO4
-0.3
-0.0
N.D.
+0.4
+0.5
carbon dioxide
growth in media lacking HCO3 buffered with 10 mM TES pH8
+0.4
+0.1
N.D.
+0.5
-0.3
carbon dioxide
growth in media lacking HCO3 buffered with 10 TES pH8 and bubbled with ambient air but diluted to prevent light attenuation
+0.1
+0.3
N.D.
+0.4
-0.1
carbon dioxide
growth in full media bubbled with ambient air
+0.4
+0.1
N.D.
-0.1
+0.3
photoheterotrophic
growth in full media and 10 mM glucose= photoheterotrophic growth but diluted to prevent slight attenuation and replinshing of glucose
+0.4
+0.2
N.D.
-0.1
+0.3
carbon dioxide
growth in media lacking HCO3 but diluted to prevent light attenuation
+0.3
+0.3
N.D.
+0.4
-0.1
carbon dioxide
growth in full media bubbled with air enriched in 5% CO2
+0.6
+0.1
N.D.
+0.3
-0.1
nitrogen source
growth in full media and 10 mM glucose= photomixotrophic growth
-0.0
+0.5
N.D.
+0.2
+0.7
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