Fitness for 5 genes in Synechocystis sp000284455 PCC 6803

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500 ntSGL_RS18470 and SGL_RS19675 are separated by 102 nucleotidesSGL_RS19675 and SGL_RS00135 are separated by 213 nucleotidesSGL_RS00135 and SGL_RS00140 overlap by 48 nucleotidesSGL_RS00140 and SGL_RS00145 are separated by 28 nucleotides SGL_RS18470: SGL_RS18470 - HNH endonuclease, at 30,281 to 30,574 _RS18470 SGL_RS19675: SGL_RS19675 - hypothetical protein, at 30,677 to 31,183 _RS19675 SGL_RS00135: SGL_RS00135 - IS701 family transposase, at 31,397 to 32,062 _RS00135 SGL_RS00140: SGL_RS00140 - ARMT1-like domain-containing protein, at 32,015 to 32,836 _RS00140 SGL_RS00145: SGL_RS00145 - DUF4079 domain-containing protein, at 32,865 to 33,350 _RS00145
Group Condition SGL_RS18470 SGL_RS19675 SGL_RS00135 SGL_RS00140 SGL_RS00145
metal stress growth in full media supplemented with 2mM sodum metaarsenite but diluted to prevent light attenuation -0.1 -0.1 N.D. +0.1 -0.5
metal stress growth in full media supplemented with 5mM sodum metaarsenite but diluted to prevent light attenuation -0.0 -0.1 N.D. -0.1 -0.6
nitrogen source NaNO2 10 mM as N source, buffered with 20 mM TES pH8 +0.1 -0.1 N.D. -0.3 -0.4
metal stress growth in full media supplemented with 3 .M Cl2Co but diluted to prevent light attenuation -0.1 -0.0 N.D. -0.1 -0.5
metal stress growth in full media supplemented with 5 .M Cl2Co +0.1 -0.1 N.D. -0.2 -0.3
metal stress growth in full media supplemented with 5mM sodum metaarsenite -0.3 -0.1 N.D. -0.1 +0.3
metal stress growth in full media supplemented with 5 .M Cl2Co -0.5 +0.1 N.D. -0.0 +0.2
nitrogen source growth in full media and 10 mM glucose= photoheterotrophic growth -0.2 -0.1 N.D. -0.2 +0.2
metal stress growth in full media supplemented with 3 .M CuSO4 +0.0 +0.2 N.D. +0.2 -0.4
metal stress growth in full media supplemented with 5mM sodum metaarsenite but diluted to prevent light attenuation -0.4 +0.1 N.D. +0.1 +0.3
nitrogen source Inoculated in media lacking a N source and incubated for 1 week, NaNo3 added and recovered for 3 days -0.2 -0.0 N.D. -0.3 +0.6
nitrogen source NaNO3 10 mM as N source, buffered with TES 20 mM TES pH8 +0.2 -0.3 N.D. -0.2 +0.4
nitrogen source NaNO3 10 mM as N source, buffered with 20 mM TES pH8 -0.0 -0.0 N.D. -0.3 +0.4
metal stress Synechocystis_PCC6803_ML3a diversity barseq (outgrowth of original library) +0.4 -0.0 N.D. -0.1 -0.1
metal stress growth in full media supplemented with 5 .M Cl2Co but diluted to prevent light attenuation -0.2 -0.2 N.D. +0.1 +0.4
nitrogen source Arg 10 mM as N source, buffered with TES 20 mM TES pH8 but diluted to prevent light attenuation -0.2 +0.0 N.D. -0.1 +0.5
glucose Na NO2 10 mM as N source, buffered with TES 20 mM TES pH8 but diluted to prevent light attenuation +0.1 -0.0 N.D. -0.2 +0.4
carbon dioxide growth in media lacking HCO3 buffered with 10 TES pH8 and bubbled with air enriched with 5% CO2 but diluted to prevent light attenuation -0.0 +0.2 N.D. +0.3 -0.2
carbon dioxide growth in full media bubbled with ambient air but diluted to prevent light attenuation +0.2 +0.3 N.D. -0.3 +0.2
carbon dioxide growth in media lacking HCO3 buffered with 10 TES pH8 and bubbled with ambient air +0.6 +0.1 N.D. -0.0 -0.2
photoheterotrophic growth in full media and 10 mM glucose= photoheterotrophic growth +0.4 -0.2 N.D. +0.0 +0.3
metal stress growth in full media supplemented with 5 .M CuSO4 -0.0 -0.0 N.D. +0.3 +0.4
metal stress growth in full media supplemented with 3 .M CuSO4 -0.3 -0.0 N.D. +0.4 +0.5
carbon dioxide growth in media lacking HCO3 buffered with 10 mM TES pH8 +0.4 +0.1 N.D. +0.5 -0.3
carbon dioxide growth in media lacking HCO3 buffered with 10 TES pH8 and bubbled with ambient air but diluted to prevent light attenuation +0.1 +0.3 N.D. +0.4 -0.1
carbon dioxide growth in full media bubbled with ambient air +0.4 +0.1 N.D. -0.1 +0.3
photoheterotrophic growth in full media and 10 mM glucose= photoheterotrophic growth but diluted to prevent slight attenuation and replinshing of glucose +0.4 +0.2 N.D. -0.1 +0.3
carbon dioxide growth in media lacking HCO3 but diluted to prevent light attenuation +0.3 +0.3 N.D. +0.4 -0.1
carbon dioxide growth in full media bubbled with air enriched in 5% CO2 +0.6 +0.1 N.D. +0.3 -0.1
nitrogen source growth in full media and 10 mM glucose= photomixotrophic growth -0.0 +0.5 N.D. +0.2 +0.7
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