Fitness for 5 genes in Cupriavidus basilensis FW507-4G11

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500 ntRR42_RS31990 and RR42_RS31995 are separated by 99 nucleotidesRR42_RS31995 and RR42_RS32000 are separated by 58 nucleotidesRR42_RS32000 and RR42_RS32005 are separated by 19 nucleotidesRR42_RS32005 and RR42_RS32010 are separated by 121 nucleotides RR42_RS31990: RR42_RS31990 - delta 4, 5-alpha steroid dehydrogenase, at 2,554,517 to 2,556,223 _RS31990 RR42_RS31995: RR42_RS31995 - flavin reductase, at 2,556,323 to 2,556,841 _RS31995 RR42_RS32000: RR42_RS32000 - 3-oxosteroid 1-dehydrogenase, at 2,556,900 to 2,558,642 _RS32000 RR42_RS32005: RR42_RS32005 - flavin-dependent monooxygenase, at 2,558,662 to 2,559,855 _RS32005 RR42_RS32010: RR42_RS32010 - 5,10-methylene tetrahydromethanopterin reductase, at 2,559,977 to 2,561,128 _RS32010
Group Condition RR42_RS31990 RR42_RS31995 RR42_RS32000 RR42_RS32005 RR42_RS32010
lb LB +0.0 -1.0 -2.3 -0.1 -0.2
stress R2A with Lomefloxacin 0.001 mM +0.2 -0.5 -1.0 -0.1 -0.9
carbon source Carbon source 2-Deoxyadenosine 5-monophosphate 5 mM -0.1 -1.3 +0.3 -0.5 -0.2
stress R2A with Chloride 300 mM +0.1 -1.4 +0.0 -0.4 +0.1
r2a R2A +0.0 -1.4 -0.1 -0.1 +0.3
stress R2A with Doxycycline hyclate 0.0001 mg/ml -0.2 -0.4 -0.8 +0.1 -0.1
stress R2A with Cobalt chloride 0.5 mM -0.6 +0.5 -0.6 -0.6 +0.0
carbon source Heptanoic (C) +0.2 -0.3 -0.6 -0.2 +0.2
stress R2A with Sisomicin 0.035 mg/ml -0.1 +0.7 -0.3 -0.3 -0.6
stress R2A with Lomefloxacin 0.00075 mM -0.6 -0.5 +0.0 -0.0 +0.4
carbon source Protocatechuic Acid (C) -0.1 -1.0 +0.2 -0.4 +0.5
carbon source Octanoic (C) +0.4 -1.0 +0.3 -0.3 +0.3
carbon source Octanoic (C) +0.2 -0.4 +0.3 -0.4 +0.3
carbon source D,L-Lactate (C) +0.4 -0.5 +0.1 -0.5 +0.4
nitrogen source L-Glutamine (N) +0.5 -1.0 +0.3 -0.1 +0.8
carbon source Carbon source 2-Deoxy-D-Ribose 20 mM -0.2 +1.0 -0.0 -0.3 +0.2
carbon source succinate (C) +0.4 +0.7 -0.0 -0.3 +0.1
carbon source Azelaic (C) +0.5 +0.2 +0.5 -0.3 +0.1
nitrogen source D-Alanine (N) +0.3 +0.6 -0.2 +0.4 -0.1
carbon source Pimelic (C) +0.2 +0.9 +0.3 -0.4 +0.2
carbon source Carbon source 2-Deoxyadenosine 5-monophosphate 5 mM +0.2 +0.7 +0.3 -0.3 +0.5
nitrogen source L-Lysine (N) +0.1 +0.6 -0.0 +0.3 +0.4
carbon source L-Leucine (C) +0.2 +0.5 +0.4 +0.2 +0.3
carbon source Azelaic (C) +0.1 +0.6 +0.5 +0.0 +0.3
carbon source D-Gluconic Acid (C) +0.3 +0.7 +0.0 +0.2 +0.2
nitrogen source L-Leucine (N) +0.3 +0.5 +0.3 +0.3 +0.2
carbon source pyruvate (C) +0.1 +0.6 +0.2 +0.4 +0.3
carbon source L-Glutamine (C) +0.2 +0.6 +0.2 +0.4 +0.2
stress R2A with Paraquat dichloride 0.064 mg/ml +0.1 +1.0 +0.2 +0.3 +0.3
carbon source L-Phenylalanine (C) +0.1 +0.6 +0.5 +0.5 +0.4
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