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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E2
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500 nt
Pf6N2E2_987 and Pf6N2E2_988 are separated by 247 nucleotides
Pf6N2E2_988 and Pf6N2E2_989 are separated by 106 nucleotides
Pf6N2E2_989 and Pf6N2E2_990 are separated by 63 nucleotides
Pf6N2E2_990 and Pf6N2E2_991 are separated by 27 nucleotides
Pf6N2E2_987 - hypothetical protein, at 1,155,380 to 1,155,616
_987
Pf6N2E2_988 - 4-hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27), at 1,155,864 to 1,156,721
_988
Pf6N2E2_989 - Transcriptional regulator, LysR family, at 1,156,828 to 1,157,742
_989
Pf6N2E2_990 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 1,157,806 to 1,158,600
_990
Pf6N2E2_991 - Ribose ABC transporter, periplasmic ribose-binding protein RbsB (TC 3.A.1.2.1), at 1,158,628 to 1,159,590
_991
Group
Condition
Pf6N2E2
_987
Pf6N2E2
_988
Pf6N2E2
_989
Pf6N2E2
_990
Pf6N2E2
_991
stress
RCH2 defined with Cobalt chloride 0.04 mM
-0.8
-1.0
-0.1
-0.4
-0.5
stress
Fluorophosphate 0.125 M
-1.4
-0.5
-0.0
-0.5
-0.1
stress
Nalidixic 0.004 mg/ml
-2.1
-0.2
-0.2
-0.1
+0.0
stress
chromate 0.000005 M
-1.6
-0.8
-0.0
+0.0
-0.1
stress
thiosulfate 0.25 M
-2.4
+0.4
-0.2
-0.2
+0.1
nitrogen source
L-Glutamic (N)
-1.8
-0.4
+0.1
+0.0
-0.3
denitrifying
Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B
-1.8
-0.2
-0.0
-0.1
+0.0
carbon source
D-Trehalose (C)
-1.6
+0.0
-0.3
+0.1
-0.2
carbon source
RCH2_defined
-1.6
+0.6
-0.4
-0.2
-0.4
nitrogen source
L-Serine (N)
-0.8
-1.0
-0.1
+0.5
-0.3
motility
outer cut, LB soft agar motility assay
-1.9
+0.1
+0.1
-0.2
+0.3
carbon source
RCH2_defined
-1.6
+0.4
-0.2
-0.3
+0.3
stress
Bile salts 0.0125 M
-1.4
+0.7
-0.1
+0.3
-0.5
stress
RCH2 defined with Zinc 0.032 mM
-1.7
+0.0
+0.2
+0.1
+0.5
stress
RCH2 defined with Cobalt chloride 0.02 mM
-1.3
+0.7
-0.2
+0.2
-0.2
stress
sodium iodate 0.005 M
-1.4
+0.9
+0.1
-0.3
-0.1
stress
arsenate 0.0005 M
+1.2
-0.8
+0.2
-0.0
+0.2
motility
inner cut, LB soft agar motility assay
+1.0
-0.4
+0.1
-0.6
+0.7
nitrogen source
nitrogen source nitrite 0.5mM
+1.6
+0.3
-0.0
+0.0
-0.9
nitrogen source
nitrogen source nitrite 1mM
+1.8
-0.5
-0.1
-0.2
+0.2
nitrogen source
nitrogen source nitrite 0.5mM
+1.8
-0.4
+0.1
-0.4
+0.2
carbon source
D-Galactose (C)
+1.2
+0.3
-0.2
+0.6
-0.1
lb
LB no stress 24 hours
+1.6
+0.2
+0.0
-0.2
+0.2
stress
Nickel (II) chloride 1.5 mM
+1.1
+0.8
+0.2
+0.3
-0.2
stress
Nickel (II) chloride 1 mM
+1.2
+0.8
+0.1
+0.1
+0.0
stress
Cobalt chloride 0.6 mM
+2.2
+0.5
+0.1
-0.5
+0.0
stress
Nickel (II) chloride 1.5 mM
+2.0
-0.4
-0.0
+0.5
+0.5
stress
Cobalt chloride 0.6 mM
+2.5
+0.2
+0.0
+0.1
-0.0
stress
Cobalt chloride 0.4 mM
+1.9
+0.5
+0.0
+0.4
+0.2
stress
Cobalt chloride 0.6 mM
+2.2
+1.2
+0.0
+0.3
-0.1
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