Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E2

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500 ntPf6N2E2_896 and Pf6N2E2_897 are separated by 21 nucleotidesPf6N2E2_897 and Pf6N2E2_898 are separated by 24 nucleotidesPf6N2E2_898 and Pf6N2E2_899 are separated by 449 nucleotidesPf6N2E2_899 and Pf6N2E2_900 are separated by 1452 nucleotides Pf6N2E2_896 - Regulatory protein, LysR:LysR, substrate-binding, at 1,049,160 to 1,050,077 _896 Pf6N2E2_897 - Maleate cis-trans isomerase (EC 5.2.1.1), at 1,050,099 to 1,050,851 _897 Pf6N2E2_898 - putative major facilitator superfamily (MFS) transporter, at 1,050,876 to 1,052,195 _898 Pf6N2E2_899 - hypothetical protein, at 1,052,645 to 1,052,776 _899 Pf6N2E2_900 - hypothetical protein, at 1,054,229 to 1,054,342 _900
Group Condition Pf6N2E2_896 Pf6N2E2_897 Pf6N2E2_898 Pf6N2E2_899 Pf6N2E2_900
motility inner cut, LB soft agar motility assay -0.6 -0.7 -0.5 N.D. N.D.
carbon source octanoate (C) -1.0 -0.5 -0.2 N.D. N.D.
denitrifying Nitrate reduction, replicate B -0.9 -0.4 -0.0 N.D. N.D.
stress Rifampicin 0.003 mg/ml -0.9 -0.4 -0.0 N.D. N.D.
stress Nickel (II) chloride 1.5 mM -1.0 +0.0 -0.2 N.D. N.D.
lb LB no stress 48 hours -1.0 +0.1 -0.2 N.D. N.D.
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A -0.5 -0.5 +0.0 N.D. N.D.
stress Nalidixic 0.004 mg/ml -0.7 +0.3 -0.6 N.D. N.D.
nitrogen source Gly-Glu (N) -0.6 -0.3 +0.1 N.D. N.D.
stress Bile salts 0.0125 M -1.3 +0.8 -0.1 N.D. N.D.
carbon source octanoate (C) -0.3 +0.2 -0.5 N.D. N.D.
stress selenate 0.5 M -0.7 +0.3 -0.1 N.D. N.D.
stress periodate 0.00125 M -0.3 +0.4 -0.3 N.D. N.D.
nitrogen source nitrogen source nitrate 5mM -0.4 +0.5 -0.4 N.D. N.D.
stress Chloride 0.5 M -0.4 +0.5 -0.3 N.D. N.D.
stress DEANONOate 0.000625 M +0.2 +0.3 -0.6 N.D. N.D.
stress Nickel (II) chloride 1 mM +0.2 -0.6 +0.4 N.D. N.D.
nitrogen source nitrogen source nitrite 1mM +0.6 -0.5 +0.1 N.D. N.D.
carbon source Ying_sugar7 rep B; time point 2 -0.3 -0.1 +0.7 N.D. N.D.
stress RCH2 defined with Cobalt chloride 0.04 mM +0.8 -0.3 -0.1 N.D. N.D.
carbon source D-Lactate (C) +0.5 +0.4 -0.3 N.D. N.D.
stress Manganese (II) chloride tetrahydrate 0.00078125 M +0.9 -0.1 -0.1 N.D. N.D.
nitrogen source nitrogen source nitrite 5mM +0.5 +0.4 -0.2 N.D. N.D.
stress Nickel (II) chloride 1 mM +0.8 -0.2 +0.2 N.D. N.D.
stress RCH2 defined with Nickel (II) chloride 0.04 mM +0.5 +0.4 -0.1 N.D. N.D.
stress RCH2 defined with Cobalt chloride 0.02 mM +0.7 +0.3 +0.1 N.D. N.D.
nitrogen source nitrogen source nitrite 5mM +1.0 +0.5 -0.3 N.D. N.D.
stress Nickel (II) chloride 1 mM +0.8 +0.2 +0.2 N.D. N.D.
carbon source RCH2_defined +0.5 +0.6 +0.1 N.D. N.D.
stress RCH2 defined with copper (II) chloride 0.03 mM +1.3 +0.5 -0.1 N.D. N.D.
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