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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E2
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500 nt
Pf6N2E2_896 and Pf6N2E2_897 are separated by 21 nucleotides
Pf6N2E2_897 and Pf6N2E2_898 are separated by 24 nucleotides
Pf6N2E2_898 and Pf6N2E2_899 are separated by 449 nucleotides
Pf6N2E2_899 and Pf6N2E2_900 are separated by 1452 nucleotides
Pf6N2E2_896 - Regulatory protein, LysR:LysR, substrate-binding, at 1,049,160 to 1,050,077
_896
Pf6N2E2_897 - Maleate cis-trans isomerase (EC 5.2.1.1), at 1,050,099 to 1,050,851
_897
Pf6N2E2_898 - putative major facilitator superfamily (MFS) transporter, at 1,050,876 to 1,052,195
_898
Pf6N2E2_899 - hypothetical protein, at 1,052,645 to 1,052,776
_899
Pf6N2E2_900 - hypothetical protein, at 1,054,229 to 1,054,342
_900
Group
Condition
Pf6N2E2
_896
Pf6N2E2
_897
Pf6N2E2
_898
Pf6N2E2
_899
Pf6N2E2
_900
motility
inner cut, LB soft agar motility assay
-0.6
-0.7
-0.5
N.D.
N.D.
carbon source
octanoate (C)
-1.0
-0.5
-0.2
N.D.
N.D.
denitrifying
Nitrate reduction, replicate B
-0.9
-0.4
-0.0
N.D.
N.D.
stress
Rifampicin 0.003 mg/ml
-0.9
-0.4
-0.0
N.D.
N.D.
stress
Nickel (II) chloride 1.5 mM
-1.0
+0.0
-0.2
N.D.
N.D.
lb
LB no stress 48 hours
-1.0
+0.1
-0.2
N.D.
N.D.
denitrifying
Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
-0.5
-0.5
+0.0
N.D.
N.D.
stress
Nalidixic 0.004 mg/ml
-0.7
+0.3
-0.6
N.D.
N.D.
nitrogen source
Gly-Glu (N)
-0.6
-0.3
+0.1
N.D.
N.D.
stress
Bile salts 0.0125 M
-1.3
+0.8
-0.1
N.D.
N.D.
carbon source
octanoate (C)
-0.3
+0.2
-0.5
N.D.
N.D.
stress
selenate 0.5 M
-0.7
+0.3
-0.1
N.D.
N.D.
stress
periodate 0.00125 M
-0.3
+0.4
-0.3
N.D.
N.D.
nitrogen source
nitrogen source nitrate 5mM
-0.4
+0.5
-0.4
N.D.
N.D.
stress
Chloride 0.5 M
-0.4
+0.5
-0.3
N.D.
N.D.
stress
DEANONOate 0.000625 M
+0.2
+0.3
-0.6
N.D.
N.D.
stress
Nickel (II) chloride 1 mM
+0.2
-0.6
+0.4
N.D.
N.D.
nitrogen source
nitrogen source nitrite 1mM
+0.6
-0.5
+0.1
N.D.
N.D.
carbon source
Ying_sugar7 rep B; time point 2
-0.3
-0.1
+0.7
N.D.
N.D.
stress
RCH2 defined with Cobalt chloride 0.04 mM
+0.8
-0.3
-0.1
N.D.
N.D.
carbon source
D-Lactate (C)
+0.5
+0.4
-0.3
N.D.
N.D.
stress
Manganese (II) chloride tetrahydrate 0.00078125 M
+0.9
-0.1
-0.1
N.D.
N.D.
nitrogen source
nitrogen source nitrite 5mM
+0.5
+0.4
-0.2
N.D.
N.D.
stress
Nickel (II) chloride 1 mM
+0.8
-0.2
+0.2
N.D.
N.D.
stress
RCH2 defined with Nickel (II) chloride 0.04 mM
+0.5
+0.4
-0.1
N.D.
N.D.
stress
RCH2 defined with Cobalt chloride 0.02 mM
+0.7
+0.3
+0.1
N.D.
N.D.
nitrogen source
nitrogen source nitrite 5mM
+1.0
+0.5
-0.3
N.D.
N.D.
stress
Nickel (II) chloride 1 mM
+0.8
+0.2
+0.2
N.D.
N.D.
carbon source
RCH2_defined
+0.5
+0.6
+0.1
N.D.
N.D.
stress
RCH2 defined with copper (II) chloride 0.03 mM
+1.3
+0.5
-0.1
N.D.
N.D.
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