Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E2

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500 ntPf6N2E2_874 and Pf6N2E2_875 are separated by 60 nucleotidesPf6N2E2_875 and Pf6N2E2_876 overlap by 4 nucleotidesPf6N2E2_876 and Pf6N2E2_877 are separated by 19 nucleotidesPf6N2E2_877 and Pf6N2E2_878 are separated by 76 nucleotides Pf6N2E2_874 - Predicted cobalt transporter CbtC, at 1,024,468 to 1,025,145 _874 Pf6N2E2_875 - putative metal chaperone, involved in Fe-nitrile hydratase activation, GTPase of COG0523 family, at 1,025,206 to 1,025,646 _875 Pf6N2E2_876 - Cobalt-containing nitrile hydratase subunit beta (EC 4.2.1.84), at 1,025,643 to 1,026,296 _876 Pf6N2E2_877 - Cobalt-containing nitrile hydratase subunit alpha (EC 4.2.1.84), at 1,026,316 to 1,026,948 _877 Pf6N2E2_878 - Amidase clustered with urea ABC transporter and nitrile hydratase functions, at 1,027,025 to 1,028,539 _878
Group Condition Pf6N2E2_874 Pf6N2E2_875 Pf6N2E2_876 Pf6N2E2_877 Pf6N2E2_878
carbon source D-Glucosamine Hydrochloride (C) -0.4 -0.4 -0.9 +0.1 -0.1
nitrogen source nitrogen source nitrate 20mM -0.1 -0.1 -0.9 -0.3 +0.1
stress sodium iodate 0.005 M -0.0 -0.6 -0.6 -0.0 +0.0
motility inner cut, LB soft agar motility assay +0.0 -0.2 -0.6 -0.2 -0.2
nitrogen source L-Lysine (N) -0.1 -0.1 -0.7 -0.0 -0.2
stress Chloride 0.5 M +0.1 -0.3 -0.4 -0.2 -0.2
carbon source octanoate (C) -0.2 -0.3 -0.7 +0.1 +0.0
nitrogen source L-Aspartic Acid (N) -0.3 +0.2 -0.5 -0.2 -0.2
lb LB no stress 48 hours +0.1 -0.2 -0.5 -0.3 -0.0
nitrogen source L-Glutamic (N) +0.1 -0.3 -0.3 -0.3 -0.1
nitrogen source L-Alanine (N) +0.1 -0.0 -0.8 -0.0 -0.1
stress Hydroxylamine 0.5 M -0.0 +0.3 -0.2 -0.6 -0.3
nitrogen source Cytosine (N) +0.3 -0.3 -0.6 -0.1 -0.1
motility outer cut, LB soft agar motility assay -0.2 -0.4 +0.3 -0.3 -0.2
nitrogen source Glucuronamide (N) +0.2 -0.2 -0.7 +0.2 -0.3
nitrogen source nitrogen source nitrite 5mM -0.0 -0.2 -0.7 +0.2 +0.0
stress DEANONOate 0.000625 M -0.2 +0.2 -0.3 -0.6 +0.1
vitamin With vitamins t2 -0.2 +0.5 -0.7 -0.2 +0.0
nitrogen source L-Isoleucine (N) +0.2 -0.3 -0.7 +0.2 -0.1
stress Nickel (II) chloride 0.0025 M +0.2 -0.1 -0.4 -0.3 +0.1
lb LB no stress 48 hours +0.1 +0.1 -0.8 +0.1 +0.2
stress molybdate 0.25 M +0.1 -0.4 +0.3 -0.0 -0.3
carbon source 4-Hydroxybenzoic Acid (C) -0.2 +0.4 -0.5 +0.0 -0.1
stress DEANONOate 0.000625 M -0.0 +0.5 -0.7 -0.0 +0.0
nitrogen source L-Serine (N) -0.6 -0.0 +0.1 +0.3 +0.2
carbon source Ying_sugar7 rep B; time point 2 +0.1 +0.5 +0.2 -0.4 -0.3
stress Bile salts 0.0125 M +0.3 +0.2 -0.4 +0.4 +0.0
stress deoxycholate 0.0125 M +0.4 -0.1 +0.4 -0.3 +0.2
resistance 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine -0.2 -0.1 +0.5 +0.5 +0.1
vitamin No vitamins t2 -0.2 +0.2 +0.4 +0.5 +0.1
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