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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E2
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500 nt
Pf6N2E2_755 and Pf6N2E2_756 are separated by 373 nucleotides
Pf6N2E2_756 and Pf6N2E2_757 are separated by 274 nucleotides
Pf6N2E2_757 and Pf6N2E2_758 overlap by 4 nucleotides
Pf6N2E2_758 and Pf6N2E2_759 are separated by 8 nucleotides
Pf6N2E2_755 - UPF0141 membrane protein YijP possibly required for phosphoethanolamine modification of lipopolysaccharide, at 864,399 to 866,138
_755
Pf6N2E2_756 - hypothetical protein, at 866,512 to 866,685
_756
Pf6N2E2_757 - 6-phosphogluconate dehydrogenase, decarboxylating (EC 1.1.1.44), at 866,960 to 867,940
_757
Pf6N2E2_758 - Glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49), at 867,937 to 869,457
_758
Pf6N2E2_759 - HMP-PP hydrolase (pyridoxal phosphatase) Cof, detected in genetic screen for thiamin metabolic genes (PMID:15292217), at 869,466 to 870,275
_759
Group
Condition
Pf6N2E2
_755
Pf6N2E2
_756
Pf6N2E2
_757
Pf6N2E2
_758
Pf6N2E2
_759
stress
Cobalt chloride 0.6 mM
-0.2
-1.4
-0.1
-0.6
-1.0
stress
Aluminum chloride 2 mM
-2.5
-0.1
-0.2
+0.1
-0.1
stress
Zinc chloride 0.0000015625 M
-2.5
+0.3
-0.8
-0.0
+0.2
carbon source
Ying_sugar7 rep C; time point 1
-0.5
-1.3
-0.3
-0.1
-0.6
stress
molybdate 0.25 M
+0.3
-1.3
-0.6
-0.5
-0.0
stress
tungstate 0.05 M
-0.3
-1.8
-0.1
+0.1
+0.1
stress
Aluminum chloride 4 mM
-1.8
-0.4
+0.2
+0.2
-0.2
carbon source
D-Galactose (C)
-1.4
+0.1
-0.4
-0.2
-0.0
carbon source
D-Galactose (C)
-1.0
-0.3
-0.1
-0.1
-0.1
carbon source
Ying_OrganicAcid7 rep C; time point 3
-0.1
-1.2
-0.3
-0.1
+0.0
stress
dimethyl sulfone 0.25 M
-0.1
-1.0
-0.1
-0.3
-0.0
stress
Fluorophosphate 0.125 M
+0.1
-1.0
-0.3
-0.3
+0.1
stress
thiosulfate 0.25 M
+0.1
-0.8
-0.2
-0.3
-0.2
stress
tungstate 0.05 M
+0.1
-1.4
-0.2
-0.0
+0.1
stress
Fluorophosphate 0.125 M
+0.2
-0.3
-0.9
-0.2
-0.1
carbon source
Ying_AminoAcid20 rep B; time point 3
+0.1
-0.7
-0.5
-0.1
+0.1
stress
dimethyl sulfone 0.25 M
+0.1
-0.8
+0.2
-0.1
-0.3
carbon source
D-Trehalose (C)
+0.4
-0.1
-0.2
-0.2
-0.6
stress
Aluminum chloride 0.00005 M
-0.2
-1.0
+0.3
+0.0
+0.1
nitrogen source
Gly-Glu (N)
+0.3
+0.2
-0.4
-0.1
-0.4
stress
copper (II) chloride 3 mM
+0.1
-0.3
+0.5
-0.2
-0.4
stress
Phosphomycin 0.004 mg/ml
-0.1
-0.0
+0.8
-0.1
-0.8
nitrogen source
L-Proline (N)
+0.9
+0.2
-0.5
+0.0
-0.1
carbon source
Ying_sugar7 rep B; time point 2
-0.7
+0.1
+0.8
-0.0
+0.4
stress
Bile salts 0.0125 M
+0.1
-0.5
+0.8
+0.1
+0.1
nitrogen source
Cytidine (N)
+0.7
+0.6
-0.1
-0.2
-0.2
nitrogen source
Cytidine (N)
+0.7
+0.2
+0.3
+0.1
-0.5
stress
Rifampicin 0.003 mg/ml
-0.4
+0.4
+1.0
-0.1
+0.1
stress
DEANONOate 0.000625 M
-0.1
+0.3
+0.3
-0.1
+0.6
stress
Doxycycline hyclate 0.0001 mg/ml
+0.3
+1.1
+1.5
+0.2
-0.0
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