Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E2

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500 ntPf6N2E2_5720 and Pf6N2E2_5721 are separated by 872 nucleotidesPf6N2E2_5721 and Pf6N2E2_5722 overlap by 1 nucleotidesPf6N2E2_5722 and Pf6N2E2_5723 are separated by 100 nucleotidesPf6N2E2_5723 and Pf6N2E2_5724 are separated by 113 nucleotides Pf6N2E2_5720 - BarA sensory histidine kinase (= VarS = GacS), at 6,533,821 to 6,536,676 _5720 Pf6N2E2_5721 - Cysteine synthase B (EC 2.5.1.47), at 6,537,549 to 6,538,451 _5721 Pf6N2E2_5722 - 23S rRNA (Uracil-5-) -methyltransferase RumA (EC 2.1.1.-) ## LSU rRNA m(5)U1939, at 6,538,451 to 6,539,821 _5722 Pf6N2E2_5723 - GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2), at 6,539,922 to 6,542,168 _5723 Pf6N2E2_5724 - Nucleoside triphosphate pyrophosphohydrolase MazG (EC 3.6.1.8), at 6,542,282 to 6,543,115 _5724
Group Condition Pf6N2E2_5720 Pf6N2E2_5721 Pf6N2E2_5722 Pf6N2E2_5723 Pf6N2E2_5724
carbon source octanoate (C) -4.3 N.D. +0.0 -4.1 -0.3
carbon source octanoate (C) -3.2 N.D. +0.2 -4.3 -0.1
carbon source L-Alanine (C) -2.7 N.D. +0.6 -4.0 -0.3
stress copper (II) chloride 3 mM -0.0 N.D. -0.7 -5.0 -0.4
carbon source L-Isoleucine (C) -0.5 N.D. -0.4 -4.8 -0.2
carbon source L-Alanine (C) -2.4 N.D. +0.5 -3.8 -0.3
stress Hydroxylamine 0.5 M -2.8 N.D. +0.7 -3.6 +0.1
stress pyrophosphate 0.0025 M -3.1 N.D. +0.4 -2.5 -0.2
carbon source L-Arabinose (C) +0.6 N.D. -0.8 -4.2 -0.1
carbon source D-Alanine (C) -4.7 N.D. +0.3 +1.1 -0.3
nitrogen source L-Alanine (N) +1.3 N.D. -1.2 -3.5 +0.0
nitrogen source L-Isoleucine (N) +1.4 N.D. -0.6 -3.9 -0.2
nitrogen source L-Isoleucine (N) +1.7 N.D. -0.0 -4.3 -0.0
carbon source propionate (C) +3.4 N.D. -0.2 -5.1 -0.1
carbon source propionate (C) +2.7 N.D. +0.0 -4.6 -0.2
stress Zinc chloride 0.0000015625 M +2.1 N.D. -0.4 -3.1 -0.6
carbon source D-Galactose (C) -3.7 N.D. +2.7 -0.4 -0.4
nitrogen source L-Alanine (N) +2.0 N.D. -0.8 -3.0 +0.1
carbon source D-Galactose (C) -3.2 N.D. +2.3 -0.1 -0.5
carbon source Carnitine Hydrochloride (C) +2.2 N.D. -0.4 -3.2 +0.2
stress DEANONOate 0.000625 M +2.8 N.D. -1.1 -2.3 -0.0
carbon source Carnitine Hydrochloride (C) +3.2 N.D. -0.5 -3.1 +0.1
stress DEANONOate 0.000625 M +3.4 N.D. -1.6 -1.4 -0.2
nitrogen source L-Serine (N) +4.8 N.D. -0.8 -3.2 -0.2
nitrogen source L-Serine (N) +4.9 N.D. -0.2 -3.1 +0.1
motility inner cut, LB soft agar motility assay +9.2 N.D. -0.2 +0.7 -0.2
motility inner cut, LB soft agar motility assay +9.9 N.D. -0.9 +0.3 +0.1
motility outer cut, LB soft agar motility assay +8.6 N.D. +0.3 +0.8 -0.2
motility inner cut, LB soft agar motility assay +10.1 N.D. -0.2 +0.3 -0.4
motility outer cut, LB soft agar motility assay +9.6 N.D. +0.2 +0.6 -0.1
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